Levy N L, Synderman R, Ladda R L, Lieberman R
Proc Natl Acad Sci U S A. 1973 Nov;70(11):3125-9. doi: 10.1073/pnas.70.11.3125.
Splenic macrophages were identified as at least one source of C5 elaboration in normal mice. Hybrid cells were formed from splenic macrophages from C5-deficient mice and either kidney cells from mice with normal amounts of C5 or chicken erythrocytes. These hybrids elaborated C5 in vitro. C5-Deficient mice inoculated with these hybrid cells developed, in their serum, antigenically active mouse C5, as well as both hemolytic and biologic complement activity. These studies demonstrate the feasibility of genetic "repair" in mammals.
脾脏巨噬细胞被确定为正常小鼠中补体C5产生的至少一个来源。杂交细胞由C5缺陷小鼠的脾脏巨噬细胞与C5含量正常的小鼠的肾细胞或鸡红细胞形成。这些杂交细胞在体外产生C5。用这些杂交细胞接种的C5缺陷小鼠的血清中出现了具有抗原活性的小鼠C5，以及溶血和生物学补体活性。这些研究证明了哺乳动物基因“修复”的可行性。
