一种从豚鼠小肠上皮细胞制备微型过氧化物酶体的区带转子法。
A zonal rotor method for the preparation of microperoxisomes from epithelial cells of guinea pig small intestine.
作者信息
Connock M J, Kirk P R, Sturdee A P
出版信息
J Cell Biol. 1974 Apr;61(1):123-33. doi: 10.1083/jcb.61.1.123.
Abstract
A method is described for the preparation of catalase particles from homogenates made from suspensions of epithelial cells of the small intestine of the guinea pig. Electron microscope examination of the preparations revealed the presence of small diaminobenzidine-positive particles measuring 0.1-0.3 nm in diameter and resembling the microperoxisomes observed by Novikoff and Novikoff (1972. J. Cell Biol.53:532.). Analytical data upon which the method is based are presented. The technique consisted of a rate zonal separation of microperoxisomes from large particles followed by an isopycnic separation from less dense organelles. Application of the method yielded microperoxisomes purified between 20- and 30-fold.
摘要
本文描述了一种从豚鼠小肠上皮细胞悬液匀浆中制备过氧化氢酶颗粒的方法。对制备物进行电子显微镜检查发现，存在直径为0.1 - 0.3纳米的小的二氨基联苯胺阳性颗粒，类似于诺维科夫和诺维科夫（1972年，《细胞生物学杂志》53:532）观察到的微过氧化物酶体。文中给出了该方法所依据的分析数据。该技术包括从大颗粒中对微过氧化物酶体进行速率区带分离，然后从不太致密的细胞器中进行等密度分离。应用该方法得到了纯化20至30倍的微过氧化物酶体。
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