Baur G, Wendel A
J Cancer Res Clin Oncol. 1980;97(3):267-73. doi: 10.1007/BF00405778.
The major parameters of the peroxide-metabolizing system were investigated in eight human colon carcinoma excisions and compared with apparently non-neoplastic colon tissue from the same patients. The mean malondialdehyde level as an index of lipid peroxidation was 147 pmol/mg protein in carcinoma compared to 40 pmol/mg in healthy tissue. The following relations of specific activities in carcinoma and healthy tissue were found: superoxide dismutase 3.9 micrograms/mg protein (carcinoma): 2.4 micrograms/mg (healthy), glutathione peroxidase 23 mU/mg:10 mU/mg; GSH-transferase 38 mU/mg:25 mU/mg; catalase 2.3 mK/mg:4.1 mK/mg. The mean selenium concentration in carcinoma was 36 ng/mg protein compared to 24 ng/mg. The difference between carcinoma and normal tissue showed an overall-significance of p < 0.002 for the whole set of data. No significant differences were found in GSH levels (12.6 nmol/mg protein in carcinoma: 9.4 nmol/mg in normal colon), GSSG levels (0.6 nmol/mg:0.7 nmol/mg) and in the protein content (70 +/- 23.5 mg/g wet tissue: 66 +/- 20 mg/g). It is concluded that the enhanced lipid peroxidation in tumor tissue may be due to peroxisomally generated H2O2 in conjunction with a lowered catalase activity.
在八例人类结肠癌切除样本中研究了过氧化物代谢系统的主要参数，并与同一患者的明显非肿瘤性结肠组织进行了比较。作为脂质过氧化指标的丙二醛平均水平在癌组织中为147 pmol/mg蛋白质，而在健康组织中为40 pmol/mg。发现癌组织和健康组织中特定酶活性存在以下关系：超氧化物歧化酶3.9微克/毫克蛋白质（癌组织）：2.4微克/毫克（健康组织），谷胱甘肽过氧化物酶23 mU/mg：10 mU/mg；谷胱甘肽-S-转移酶38 mU/mg：25 mU/mg；过氧化氢酶2.3 mK/mg：4.1 mK/mg。癌组织中的平均硒浓度为36 ng/mg蛋白质，而健康组织中为24 ng/mg。对于整个数据集，癌组织与正常组织之间的差异显示出总体显著性，p < 0.002。在谷胱甘肽水平（癌组织中为12.6 nmol/mg蛋白质：正常结肠中为9.4 nmol/mg）、氧化型谷胱甘肽水平（0.6 nmol/mg：0.7 nmol/mg）和蛋白质含量（70±23.5 mg/g湿组织：66±20 mg/g）方面未发现显著差异。得出的结论是，肿瘤组织中脂质过氧化增强可能是由于过氧化物酶体产生的过氧化氢与过氧化氢酶活性降低共同作用的结果。
