Johnson M C, Bozzola J J, Shechmeister I L
J Bacteriol. 1974 Apr;118(1):304-11. doi: 10.1128/jb.118.1.304-311.1974.
Two extracellular polysaccharide mutants of Streptococcus mutans GS-5 were obtained and examined. The mutants were distinguished by colonial morphology and by growth on and adherence to hard surfaces. A technique was devised which allowed these bacteria to be studied as they appeared when grown on a hard surface in liquid medium which contained sucrose. Negative stains, replicas, and scanning electron micrography clearly revealed differences in cellular aggregation due to the various extracellular polysaccharides produced. Comparison of sections of the adherent parent strain (GS-5) with those of the nonadherent mutant (GS-511) allowed the extracellular polysaccharide(s) responsible for adhesion to be visually localized.
获得并检测了变形链球菌GS-5的两个细胞外多糖突变体。这些突变体通过菌落形态以及在硬表面上的生长和黏附情况得以区分。设计了一种技术，可对这些细菌在含有蔗糖的液体培养基中的硬表面上生长时的形态进行研究。负染色、复制品和扫描电子显微镜清楚地揭示了由于产生的各种细胞外多糖而导致的细胞聚集差异。将黏附亲本菌株（GS-5）与非黏附突变体（GS-511）的切片进行比较，使得负责黏附的细胞外多糖能够在视觉上定位。
