Schneeberger E E, Leber P D, Karnovsky M J, McCluskey R T
J Exp Med. 1974 May 1;139(5):1283-302. doi: 10.1084/jem.139.5.1283.
The altered functional properties of the glomerular capillary wall in a model of autologous immune complex disease (Heymann's nephritis) was studied by electron microscopy using intravenously injected protein tracers of varying molecular weight. There was an increase in the permeability of the glomerular basement membrane (GBM) itself to large molecules; this change was focal and was found in those areas where the GBM contained immune complex deposits. Both ferritin and catalase, tracers normally restricted from passing the glomerular filter, were present in the urinary space within minutes of injection. No evidence was obtained for increased glomerular epithelial transport in this disease. Foot process swelling and "close" junction formation was moderate, even in animals with marked degrees of proteinuria. Indirect evidence, therefore, makes an alteration in the slit pore complex likely. In addition, there was immediate and selective concentration of all tracers within deposits, though ferritin was partially excluded from some deposits. This phenomenon may be of significance in the perpetuation of the disease.
利用静脉注射不同分子量的蛋白质示踪剂，通过电子显微镜研究了自体免疫复合物疾病模型（海曼肾炎）中肾小球毛细血管壁功能特性的改变。肾小球基底膜（GBM）本身对大分子的通透性增加；这种变化是局灶性的，且在GBM含有免疫复合物沉积的区域发现。注射后几分钟内，尿腔中就出现了通常被限制通过肾小球滤过的示踪剂铁蛋白和过氧化氢酶。在这种疾病中未获得肾小球上皮转运增加的证据。足突肿胀和“紧密”连接形成程度适中，即使在有明显蛋白尿程度的动物中也是如此。因此，间接证据表明裂孔复合体可能发生了改变。此外，所有示踪剂在沉积物中立即有选择性地浓缩，尽管铁蛋白部分被某些沉积物排除。这种现象可能对疾病的持续存在具有重要意义。
