从正常大鼠肾细胞分离出的液滴中酸性磷酸酶、核糖核酸酶、脱氧核糖核酸酶、β-葡萄糖醛酸酶和组织蛋白酶的浓度。
Concentration of acid phosphatase, ribonuclease, desoxyribonuclease, beta-glucuronidase, and cathepsin in droplets isolated from the kidney cells of normal rats.
作者信息
STRAUS W
出版信息
J Biophys Biochem Cytol. 1956 Sep 25;2(5):513-21. doi: 10.1083/jcb.2.5.513.
Abstract
- Three fractions of "droplets" having diameters of 1 to 5 micro (fraction I), 0.5 to 1.5 micro (fraction II), and 0.1 to 1.0 micro (fraction III) were isolated from the kidney cells of normal rats. 2. All three "droplet" fractions showed 10 to 15 times higher activities of acid phosphatase, beta-glucuronidase, ribonuclease, desoxyribonuclease, and cathepsin than the total homogenate and the mitochondrial fraction. 3. After a rough fractionation of the total homogenate, approximately 50 per cent of the 5 enzymes was found in the fractions which contained the "droplets" and approximately 30 per cent in the supernatant fluid. 4. The similarities between the enzymatic properties of the "droplets" from kidney cells and of the fractions isolated from liver cells by other investigators have been discussed.
摘要
- 从正常大鼠的肾细胞中分离出了三类“液滴”，其直径分别为1至5微米（组分I）、0.5至1.5微米（组分II）和0.1至1.0微米（组分III）。2. 所有这三类“液滴”组分的酸性磷酸酶、β-葡萄糖醛酸酶、核糖核酸酶、脱氧核糖核酸酶和组织蛋白酶的活性比总匀浆和线粒体组分高10至15倍。3. 在对总匀浆进行初步分级分离后，发现约50%的这5种酶存在于含有“液滴”的组分中，约30%存在于上清液中。4. 已经讨论了肾细胞“液滴”的酶学性质与其他研究者从肝细胞中分离出的组分的酶学性质之间的相似性。
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