Vialli D M
Acta Histochem. 1977;60(1):103-20.
The author begins by dealing with a number of general questions on argentaffinity and argentophilia. He then goes on to analyze the individual phases in which the methods are performed, distinguishing and analyzing certain characteristics: a) of the materials fixation, b) of the steps preceding the argentic reaction, c) of the argentic treatment, d) of the development, e) of the fixing, f) of the toning, and g) of the after-treatments and counter-colorations. In particular, as regards the argentic treatment, he recalls some anomalous or little-known methods of impregnation, and, as regards the development, deals not only with the chemical, but also with the physical development, which is important, although less used. Next, he considers certain deductions that can be made from the results of hypo- and hypertreatments. Lastly, he discusses the possibility of comparing different methods within the framework of each type, as well as between types. In conclusion, the author recalls the value, in histochemistry, of argentaffinity and argentophilia reactions, and stresses the importance of the importance of the analysis of treatment by phases, both in order to arrive at a more precise critical evaluation of the date obtained, and with regard to possible further research.