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普氏立克次氏体细胞壁的电子显微镜观察。

Electron microscopy of the cell wall of Rickettsia prowazeki.

作者信息

Palmer E L, Mallavia L P, Tzianabos T, Obijeski J F

出版信息

J Bacteriol. 1974 Jun;118(3):1158-66. doi: 10.1128/jb.118.3.1158-1166.1974.

DOI:10.1128/jb.118.3.1158-1166.1974
PMID:4133612
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC246866/
Abstract

Purified Rickettsia prowazeki were found to undergo morphological changes resembling plasmolysis when stained with uranyl acetate, resulting in rod-like forms. Sequential electron micrographs of disintegrating organisms provide evidence for the cell wall origin of these rod-like forms. The substructure of the cell wall was discerned by using negative-contrast electron microscopy. The wall was found to be composed of repetitive subunits with a periodicity of 13 nm and was surrounded by a thin membrane.

摘要

经发现,用醋酸铀酰染色时,纯化的普氏立克次氏体发生了类似质壁分离的形态变化,形成了杆状形态。对解体生物体的连续电子显微镜照片为这些杆状形态的细胞壁起源提供了证据。通过负染色电子显微镜观察到了细胞壁的亚结构。发现细胞壁由周期为13纳米的重复亚基组成,并被一层薄膜包围。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac91/246866/063f96949d20/jbacter00342-0410-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac91/246866/32f8d154f479/jbacter00342-0405-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac91/246866/a1c1a0f68286/jbacter00342-0406-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac91/246866/2bf26b73ca1d/jbacter00342-0406-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac91/246866/cd3882359551/jbacter00342-0407-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac91/246866/2580b59bf337/jbacter00342-0408-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac91/246866/a1d09b69851a/jbacter00342-0409-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac91/246866/067743dabbfd/jbacter00342-0409-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac91/246866/14979228d7a4/jbacter00342-0410-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac91/246866/2035ba5210ac/jbacter00342-0410-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac91/246866/063f96949d20/jbacter00342-0410-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac91/246866/32f8d154f479/jbacter00342-0405-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac91/246866/a1c1a0f68286/jbacter00342-0406-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac91/246866/2bf26b73ca1d/jbacter00342-0406-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac91/246866/cd3882359551/jbacter00342-0407-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac91/246866/2580b59bf337/jbacter00342-0408-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac91/246866/a1d09b69851a/jbacter00342-0409-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac91/246866/067743dabbfd/jbacter00342-0409-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac91/246866/14979228d7a4/jbacter00342-0410-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac91/246866/2035ba5210ac/jbacter00342-0410-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac91/246866/063f96949d20/jbacter00342-0410-c.jpg

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Cell-wall constituents of rickettsiae and psittacosis-lymphogranuloma organisms.立克次氏体和鹦鹉热-淋巴肉芽肿病原体的细胞壁成分。
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