Fischman D A, Weinbaum G
Science. 1967 Jan 27;155(3761):472-4. doi: 10.1126/science.155.3761.472.
Cell walls, isolated from Escherichia coli B, as examined by electron microscopy and optical diffraction contain a hexagonal lattice structure, the (1,0) planes of which are separated by 140 +/- 8 angstroms. Unless the walls are briefly heated (10 minutes, 90 degrees C) early in the isolation, the hexagonal array cannot always be observed. Enzymatic digestion with pancreatin and amylase improves visualization of the lattice; subsequent treatment with pepsin and sodium dodecylsulfate removes the hexagonal pattern. Protein or lipoprotein globular units within the wall may thus be arranged in a hexagonal array uponthe mucopeptide layer.
从大肠杆菌B分离得到的细胞壁，经电子显微镜和光学衍射检查，含有一种六边形晶格结构，其(1,0)平面间距为140±8埃。除非在分离初期将细胞壁短暂加热（10分钟，90摄氏度），否则六边形阵列并非总能观察到。用胰蛋白酶和淀粉酶进行酶消化可改善晶格的可视化效果；随后用胃蛋白酶和十二烷基硫酸钠处理会消除六边形图案。因此，壁内的蛋白质或脂蛋白球状单元可能在粘肽层上排列成六边形阵列。
