Fukui K, Kokeguchi S, Kato K, Miyake Y, Nogami R, Moriyama T
Infect Immun. 1983 Feb;39(2):762-6. doi: 10.1128/iai.39.2.762-766.1983.
Antiserum against purified mutansynthetase (EC 2.4.1.?) of Streptococcus mutans 6715 (serotype g), which is responsible for the synthesis of water-insoluble glucan (ISG) in the presence of both sucrose and water-soluble glucan, was prepared. The specificity of the antiserum was tested by using crude enzyme preparations (CEPs) of S. mutans strains of various serotypes. On immunodiffusion, the antiserum cross-reacted with CEPs from strains of serotypes a (HS-6 and AHT), d (OMZ176), and g (OMZ65 and KIR), but not with those from strains of serotypes b (BHT and FA-1) and c (GS-5 and Ingbritt). The antiserum inhibited the synthesis of ISG by crude or purified mutansynthetase of S. mutans 6715. The activities of ISG synthesis by CEPs from the strains antigenically related in the foregoing immunodiffusion were inhibited by the antiserum against strain 6715 mutansynthetase. The antiserum, however, also inhibited the enzyme activity of the strains of serotype b. The finding that the antiserum against purified dextransucrase of S. mutans HS-6 inhibited ISG synthesis by a CEP of strain HS-6 and also by CEPs of antigenically related strains suggested that dextransucrase activity is involved in ISG synthesis.
制备了抗变形链球菌6715（血清型g）纯化变聚糖合成酶（EC 2.4.1.?）的抗血清，该酶在蔗糖和水溶性葡聚糖存在的情况下负责合成水不溶性葡聚糖（ISG）。通过使用各种血清型变形链球菌菌株的粗酶制剂（CEP）来测试抗血清的特异性。在免疫扩散实验中，该抗血清与血清型a（HS - 6和AHT）、d（OMZ176）和g（OMZ65和KIR）菌株的CEP发生交叉反应，但与血清型b（BHT和FA - 1）和c（GS - 5和Ingbritt）菌株的CEP不发生交叉反应。该抗血清抑制了变形链球菌6715的粗酶或纯化变聚糖合成酶合成ISG的过程。在上述免疫扩散中具有抗原相关性的菌株的CEP合成ISG的活性被抗6715菌株变聚糖合成酶的抗血清所抑制。然而，该抗血清也抑制了血清型b菌株的酶活性。抗变形链球菌HS - 6纯化葡聚糖蔗糖酶的抗血清抑制HS - 6菌株的CEP以及抗原相关菌株的CEP合成ISG这一发现表明，葡聚糖蔗糖酶活性参与了ISG的合成。
