Wheatley D N, Mueller G C, Kajiwara K
Br J Cancer. 1974 Feb;29(2):117-31. doi: 10.1038/bjc.1974.48.
Bleomycin inhibits cell division in HeLa S-3 and other mammalian epithelial cell lines. Its effect on suspension cultured cells is far greater than on monolayer cells; this was found to be due partly to the absence of divalent ions. Other mammalian epithelial lines in suspension culture are very sensitive but fibroblastic cells (BHK 21/C13/DWS-3) grown under identical conditions are relatively insensitive. This study has established that bleomycin is a powerful anti-mitotic agent during the G(2) phase but it does not prevent prophase cells from completing a normal mitotic division. Its ability to suppress DNA synthesis contributes little to the inhibition of cell cycling.
博来霉素可抑制海拉S-3细胞及其他哺乳动物上皮细胞系的细胞分裂。其对悬浮培养细胞的作用远大于对单层细胞的作用；发现这部分是由于缺乏二价离子所致。悬浮培养的其他哺乳动物上皮细胞系非常敏感，但在相同条件下培养的成纤维细胞（BHK 21/C13/DWS-3）相对不敏感。本研究证实，博来霉素在G(2)期是一种强大的抗有丝分裂剂，但它并不阻止前期细胞完成正常的有丝分裂。其抑制DNA合成的能力对细胞周期抑制的作用不大。
