20分钟内完成细胞周期分析。
Cell-cycle analysis in 20 minutes.
作者信息
Crissman H A, Tobey R A
出版信息
Science. 1974 Jun 21;184(4143):1297-8. doi: 10.1126/science.184.4143.1297.
PMID:4133816
Abstract
Mithramycin added to mammalian cells fixed in aqueous ethanol is bound to DNA and fluoresces in direct proportion to the cellular DNA content. Quantitative fluorescence measurement by means of a high-speed flow system instrument provides a rapid method for cell-cycle analysis and for the first time permits continuous monitoring of cell-cycle kinetics during ongoing experiments.
摘要
将光辉霉素添加到用乙醇水溶液固定的哺乳动物细胞中，它会与DNA结合，并以与细胞DNA含量成正比的方式发出荧光。借助高速流动系统仪器进行定量荧光测量，为细胞周期分析提供了一种快速方法，并且首次能够在正在进行的实验过程中持续监测细胞周期动力学。
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