从一种B型RNA肿瘤病毒中分离并鉴定RNA指导的DNA聚合酶。
Isolation and characterization of RNA-directed DNA polymerase from a B-type RNA tumor virus.
作者信息
Dion A S, Vaidya B, Fout G S, Moore D H
出版信息
J Virol. 1974 Jul;14(1):40-6. doi: 10.1128/JVI.14.1.40-46.1974.
Abstract
RNA-directed DNA polymerase was isolated from milk-borne B-type murine mammary tumor virus of the RIII mouse strain. The several hundred-fold-purified enzyme sediments at 5.5 to 5.7S with an average molecular weight of approximately 100,000. The purified enzyme is completely template dependent and responds to RNA, DNA, and synthetic templates. Stability studies indicate differential lability dependent on the exogenous template used to monitor activity.
摘要
RNA 指导的 DNA 聚合酶是从 RIII 小鼠品系的乳汁传播的 B 型小鼠乳腺肿瘤病毒中分离出来的。经过几百倍纯化的这种酶在 5.5 至 5.7S 处沉降，平均分子量约为 100,000。纯化后的酶完全依赖模板，并对 RNA、DNA 和合成模板有反应。稳定性研究表明，其稳定性差异取决于用于监测活性的外源模板。
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