小鼠乳腺肿瘤病毒RNA分子量的电泳分析

Electrophoretic analysis of the molecular weight of murine mammary tumor virus RNA.

作者信息

Dion A S, Heine U I, Pomenti A A, Korb J, Weber G H

出版信息

J Virol. 1977 Jun;22(3):822-5. doi: 10.1128/JVI.22.3.822-825.1977.

DOI:10.1128/JVI.22.3.822-825.1977

PMID:195092

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC515781/

Abstract

Molecular weight determinations of native and subunit RNAs of murine mammary tumor virus (MuMTV), a type B oncornavirus, were performed by polyacrylamide gel electrophoresis and compared with molecular weights of well-characterized avian cellular RNAs and tobacco mosaic virus RNA. From extrapolations of semilog plots of the molecular weights of the standard RNAs versus relative electrophoretic mobilities and Ferguson plots, the subunit and native RNAs of MuMTV were found to possess molecular weights of 2.93 X 10(6) and 6.45 X 10(6), respectively. These data support the assumption that two subunit molecules comprise the native RNA of MuMTV.

摘要

对B型肿瘤病毒——鼠乳腺肿瘤病毒（MuMTV）的天然RNA和亚基RNA进行了分子量测定，采用聚丙烯酰胺凝胶电泳法，并与特征明确的禽细胞RNA和烟草花叶病毒RNA的分子量进行了比较。通过标准RNA分子量对相对电泳迁移率的半对数图外推法和弗格森图，发现MuMTV的亚基RNA和天然RNA分子量分别为2.93×10⁶和6.45×10⁶。这些数据支持了以下假设：两个亚基分子构成了MuMTV的天然RNA。