de Smet M J, Kingma J, Witholt B
Biochim Biophys Acta. 1978 Jan 4;506(1):64-80. doi: 10.1016/0005-2736(78)90435-2.
The effect of toluene on Escherichia coli has been examined. In the presence of Mg2+, toluene removes very little protein, phospholipid, or lipopolysacharide from E. coli. In the absence of Mg2+, or in the presence of EDTA, toluene removes considerably more cell material, including several specific cytoplasmic proteins such as malate dehydrogenase (EC 1.1.1.37). In contrast, glucose-6-phosphate dehydrogenase (EC 1.1.1.49) and glutamate dehydrogenase (EC 1.4.1.4) are not released at all under the same conditions. Cells treated with toluene in the presence of Mg2+ remain relatively impermeable to pyridne nucleotides, while cells treated with toluene in the presence of EDTA become permeable to these compounds. Freeze-fracture electron microscopy shows that toluene causes considerable damage to the cytoplasmic membrane, while the outer membrane remains relatively intact. These results indicate that the permeability characteristics of toluene-treated cells depend at least partly on the state of the outer membrane after the toluene treatment.
已对甲苯对大肠杆菌的影响进行了研究。在镁离子存在的情况下，甲苯从大肠杆菌中去除的蛋白质、磷脂或脂多糖极少。在没有镁离子的情况下，或在乙二胺四乙酸（EDTA）存在的情况下，甲苯去除的细胞物质要多得多，包括几种特定的细胞质蛋白，如苹果酸脱氢酶（EC 1.1.1.37）。相比之下，在相同条件下，葡萄糖-6-磷酸脱氢酶（EC 1.1.1.49）和谷氨酸脱氢酶（EC 1.4.1.4）根本不会释放出来。在镁离子存在的情况下用甲苯处理的细胞对吡啶核苷酸仍然相对不渗透，而在EDTA存在的情况下用甲苯处理的细胞对这些化合物变得具有渗透性。冷冻断裂电子显微镜显示，甲苯对细胞质膜造成了相当大的损伤，而外膜相对保持完整。这些结果表明，甲苯处理过的细胞的渗透特性至少部分取决于甲苯处理后外膜的状态。
