Trelstad R L, Hayashi K, Toole B P
J Cell Biol. 1974 Sep;62(3):815-30. doi: 10.1083/jcb.62.3.815.
The embryonic corneal epithelium synthesizes both collagen and chondroitin sulfate and excretes them across the basement membrane into the subepithelial space where they assemble into a spiraling orthogonal matrix of fibrils. The assembly of collagen into fibrils is first apparent at the outer face of the basement membrane in a region of ordered chondroitin sulfate molecules. Hyaluronate, another morphogenetically important corneal macromolecule, is produced at these early stages only by the inner endothelium. These correlated biosynthetic and ultrastructural data demonstrate discrete macromolecular products of the two corneal epithelia with differing morphogenetic properties and functions.
胚胎角膜上皮合成胶原蛋白和硫酸软骨素,并将它们穿过基底膜排泄到上皮下间隙,在那里它们组装成螺旋状的正交纤维基质。胶原蛋白组装成纤维首先在基底膜外表面一个有序硫酸软骨素分子区域变得明显。透明质酸是另一种在形态发生上重要的角膜大分子,仅在这些早期阶段由内皮层产生。这些相关的生物合成和超微结构数据表明,两种角膜上皮具有不同形态发生特性和功能的离散大分子产物。