Hay E D, Dodson J W
J Cell Biol. 1973 Apr;57(1):190-213. doi: 10.1083/jcb.57.1.190.
Corneal epithelium from 5-7-day old chick embryos was isolated with EDTA and grown in culture on frozen-killed lens as a substratum. Autoradiographs showed that in the presence of [(3)H]proline, the corneal epithelium synthesized and secreted onto the lens substratum, radioactive materials resistant to extraction by sodium hydroxide. The radioactive label was associated with newly formed striated collagen fibrils, large "sheets" of collagen, and basal lamina. The repeat period and interband pattern of the abundant new collagen sheets and fibrils was typical of "native" or so-called "mesenchymal" collagen. Collagen-like materials were observed in secretory (Golgi) vacuoles within the corneal cells and collagen fibrils within the intercellular canals (lateral interfaces) of the epithelium, as well as at the base of the cells. Both the granular endoplasmic reticulum and Golgi complexes were highly developed in the corneal epithelium. In the discussion, the role of cytoplasmic organelles in collagen secretion, the origin and structure of the basal lamina, and variations in collagen polymerization patterns in vitro are reviewed and evaluated. The morphogenetic significance of the synthesis and secretion of collagen by embryonic epithelium is appraised and the production of true native-striated collagen by epithelium is stressed.
用乙二胺四乙酸（EDTA）分离5 - 7日龄鸡胚的角膜上皮，并将其培养在冷冻杀死的晶状体上作为基质。放射自显影片显示，在存在[³H]脯氨酸的情况下，角膜上皮合成并分泌到晶状体基质上的放射性物质，这些物质对氢氧化钠提取具有抗性。放射性标记与新形成的横纹胶原纤维、大片状胶原以及基膜相关。丰富的新胶原片层和纤维的重复周期和带间模式是“天然”或所谓“间充质”胶原的典型特征。在角膜细胞内的分泌（高尔基体）泡以及上皮细胞间通道（侧面界面）内的胶原纤维中，以及细胞基部都观察到了类胶原物质。角膜上皮中的粗面内质网和高尔基体复合体都高度发达。在讨论中，对细胞质细胞器在胶原分泌中的作用、基膜的起源和结构以及体外胶原聚合模式的变化进行了综述和评估。评估了胚胎上皮合成和分泌胶原的形态发生意义，并强调了上皮产生真正的天然横纹胶原。
