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Acta Vet Scand. 1974;15(2):198-205. doi: 10.1186/BF03547481.
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本文引用的文献

1
Established eurythermic line of fish cells in vitro.已建立的鱼类细胞体外广温性细胞系。
Science. 1962 Mar 23;135(3508):1065-6. doi: 10.1126/science.135.3508.1065.
2
A permanent cell line from the fathead minnow (Pimephales promelas).一种来自黑头软口鲦鱼（肥头鲦，Pimephales promelas）的永久细胞系。
Ann N Y Acad Sci. 1965 Aug 10;126(1):555-65. doi: 10.1111/j.1749-6632.1965.tb14302.x.
3
Serological identification of Egtved virus (virus of viral haemorrhagic septicaemia of rainbow trout). A preliminary report.艾格特维德病毒（虹鳟病毒性出血性败血症病毒）的血清学鉴定。初步报告。
Bull Off Int Epizoot. 1968 Jul-Aug;69(7):985-9.
4
Research on the virus of Egtved disease.关于埃格特维德病病毒的研究。
Ann N Y Acad Sci. 1965 Aug 10;126(1):422-6. doi: 10.1111/j.1749-6632.1965.tb14292.x.
5
Egtved virus: demonstration of virus antigen by the fluorescent antibody technique in tissues of rainbow trout affected by viral haemorrhagic septicaemia and in cell cultures infected with Egtved virus.埃格特维德病毒：通过荧光抗体技术在受病毒性出血性败血症影响的虹鳟鱼组织以及感染埃格特维德病毒的细胞培养物中证明病毒抗原。
Arch Gesamte Virusforsch. 1972;36(1):115-22. doi: 10.1007/BF01250301.
6
Freund's adjuvants: their influence on the specificity of viral antisera.弗氏佐剂：它们对病毒抗血清特异性的影响。
Acta Pathol Microbiol Scand B Microbiol Immunol. 1972;80(6):931-3.
7
The nature and biological activity of IPN virus neutralizing antibodies in normal and immunized rainbow trout (Salmo gairdneri).正常及免疫虹鳟（虹鳟）体内IPN病毒中和抗体的性质及生物学活性
Arch Gesamte Virusforsch. 1973;42(1):9-20.

用于检测鲑鱼病毒及相应抗体的间接荧光抗体技术。

Indirect fluorescent antibody techniques for demonstration of trout viruses and corresponding antibody.

作者信息

Jorgensen P E

出版信息

Acta Vet Scand. 1974;15(2):198-205. doi: 10.1186/BF03547481.

DOI:10.1186/BF03547481
PMID:4136814
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8407302/
Abstract

Two variations of the indirect fluorescent antibody technique (FAT) have been utilized in the work concerning two important virus diseases of trout, viral haemorrhagic septicaemia (VHS) and infectious pancreatic necrosis (IPN). A “two layer” indirect FAT allowed demonstration of the respective viruses in cell cultures and a “three layer” indirect FAT allowed demonstration of trout antibody to the viruses. Antibody, by means of the latter technique, could be demonstrated only in artificially immunized trout.

摘要

在关于鳟鱼的两种重要病毒性疾病，即病毒性出血性败血症（VHS）和传染性胰腺坏死病（IPN）的研究工作中，采用了间接荧光抗体技术（FAT）的两种变体。“双层”间接FAT可在细胞培养物中检测到相应病毒，“三层”间接FAT则可检测到鳟鱼针对这些病毒的抗体。通过后一种技术，仅在人工免疫的鳟鱼中检测到了抗体。