用于检测鲑鱼病毒及相应抗体的间接荧光抗体技术。
Indirect fluorescent antibody techniques for demonstration of trout viruses and corresponding antibody.
作者信息
Jorgensen P E
出版信息
Acta Vet Scand. 1974;15(2):198-205. doi: 10.1186/BF03547481.
Abstract
Two variations of the indirect fluorescent antibody technique (FAT) have been utilized in the work concerning two important virus diseases of trout, viral haemorrhagic septicaemia (VHS) and infectious pancreatic necrosis (IPN). A “two layer” indirect FAT allowed demonstration of the respective viruses in cell cultures and a “three layer” indirect FAT allowed demonstration of trout antibody to the viruses. Antibody, by means of the latter technique, could be demonstrated only in artificially immunized trout.
摘要
在关于鳟鱼的两种重要病毒性疾病，即病毒性出血性败血症（VHS）和传染性胰腺坏死病（IPN）的研究工作中，采用了间接荧光抗体技术（FAT）的两种变体。“双层”间接FAT可在细胞培养物中检测到相应病毒，“三层”间接FAT则可检测到鳟鱼针对这些病毒的抗体。通过后一种技术，仅在人工免疫的鳟鱼中检测到了抗体。
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