Lake P, Sabbadini E, Sehon A H
Immunology. 1974 Sep;27(3):441-55.
To study the specificity of transplantation immunity the capacity of two aggressor cell populations from allografted mice to destroy target cells obtained from a panel of strains having different H-2 types was assessed. Chromium-labelled macrophages were found to be suitable target cells to allow for quantitative comparisons of cytotoxicity. Spleen cells from skin-grafted mice lysed only target cells of strains which shared at least one H-2 region (K or D) with the donor. By contrast, non-adherent peritoneal exudate cells, produced as a consequence of ascites tumour allografts, exhibited a cytotoxic potential approximately 16- to 32-fold greater than the above spleen cells and were capable of lysing third-party target cells of strains having disparity with the graft donor at both H-2 regions. The difference between the two systems appears to be quantitative. These findings appear to reconcile previously conflicting observations, and suggest minimal or no participation of public H-2 specificities in cell-mediated reactions.
为研究移植免疫的特异性，评估了来自同种异体移植小鼠的两个攻击细胞群体破坏从一组具有不同H-2类型的品系获得的靶细胞的能力。发现铬标记的巨噬细胞是适合进行细胞毒性定量比较的靶细胞。皮肤移植小鼠的脾细胞仅裂解与供体至少共享一个H-2区域（K或D）的品系的靶细胞。相比之下，由腹水肿瘤同种异体移植产生的非贴壁腹膜渗出细胞表现出的细胞毒性潜力比上述脾细胞大约高16至32倍，并且能够裂解在两个H-2区域与移植供体存在差异的品系的第三方靶细胞。这两个系统之间的差异似乎是定量的。这些发现似乎调和了先前相互矛盾的观察结果，并表明公共H-2特异性在细胞介导的反应中参与极少或没有参与。
