Ginsburg H
Immunology. 1968 May;14(5):621-35.
Rat lymphocytes cultured on mouse embryo cell monolayers produced large pyroninophilic cells (LPC) which lysed the mouse cells. The LPC that developed on monolayers of any particular strain of mouse (originator monolayers) were tested, by transfer, for their ability to lyse monolayers of other mouse strains. The distribution of lysis among the various strains of mouse revealed a definite pattern of specificity. Analysis of the H-2 allelic complement of the mouse strains tested suggests that the lymphocytes were sensitized upon exposure to the mouse embryo monolayers against one or more of the antigens determined by the H-2 locus. The presence or absence of one or all of the antigens in other strains determined whether monolayers of these strains were lysed completely, partially, or not at all. It was concluded that the cultures obtained are an reflection of the graft host immune reaction. It was produced in the tissue culture as a primary response by normal lymphocytes.
在小鼠胚胎细胞单层上培养的大鼠淋巴细胞产生了可裂解小鼠细胞的大嗜派洛宁细胞（LPC）。通过转移测试在任何特定品系小鼠（原始单层）的单层上形成的LPC裂解其他小鼠品系单层的能力。各小鼠品系间裂解分布呈现出明确的特异性模式。对所测试小鼠品系的H-2等位基因互补情况的分析表明，淋巴细胞在接触小鼠胚胎单层时针对由H-2位点决定的一种或多种抗原致敏。其他品系中一种或所有抗原的存在与否决定了这些品系的单层是被完全裂解、部分裂解还是根本不被裂解。得出的结论是，所获得的培养物反映了移植物-宿主免疫反应。它是在组织培养中由正常淋巴细胞产生的一种初级反应。
