通过冷冻蚀刻和负染色技术鉴定叶绿体偶联因子。
Identification of chloroplast coupling factor by freeze-etching and negative-staining techniques.
作者信息
Garber M P, Steponkus P L
出版信息
J Cell Biol. 1974 Oct;63(1):24-34. doi: 10.1083/jcb.63.1.24.
Abstract
Identification of chloroplast coupling factor particles, by the freeze-etching and negative-staining techniques, was made utilizing chloroplast thylakoids isolated from spinach leaves. Complete removal of particles, comparable in diameter to purified coupling factor particles, from the outer surface of freeze-etched thylakoids was achieved by treatment with 0.8% silicotungstate. Reappearance of particles, comparable in diameter to purified coupling factor particles, on the outer surface of freeze-etched thylakoids was demonstrated by combining silicotungstate-treated thylakoids with purified chloroplast coupling factor. Negative-staining results were in agreement with the freeze-etch data. The results demonstrate that the chloroplast coupling factor particles are exposed on the outer surface.
摘要
利用从菠菜叶中分离出的叶绿体类囊体，通过冷冻蚀刻和负染色技术对叶绿体偶联因子颗粒进行了鉴定。用0.8%的硅钨酸盐处理冷冻蚀刻类囊体的外表面，可完全去除直径与纯化的偶联因子颗粒相当的颗粒。将经硅钨酸盐处理的类囊体与纯化的叶绿体偶联因子结合，证明冷冻蚀刻类囊体的外表面出现了直径与纯化的偶联因子颗粒相当的颗粒。负染色结果与冷冻蚀刻数据一致。结果表明，叶绿体偶联因子颗粒暴露在外表面。
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