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一种评估同种异体移植灭菌程序对人成纤维细胞活力影响的方法。

A method for evaluating the effects of allograft sterilization procedures on the viability of human fibroblasts.

作者信息

Chalcroft S C, Gavin J B, Steelye R N

出版信息

Thorax. 1974 Sep;29(5):539-44. doi: 10.1136/thx.29.5.539.

Abstract

, , 539-544. A semiquantitative method for assessing the effects of allograft sterilization procedures on the viability of cultured human fibroblasts is described. The proportions of viable', altered', and `non-viable' cells in four groups of fibroblasts on coverslips were estimated using light microscopy and their staining reactions with neutral red and nigrosin. While a control group of fibroblasts remained viable, exposure for one to three weeks to each of three solutions used in allograft preparation produced a progressive transition first to the altered and then to the non-viable category. These findings were confirmed by electron microscopic examination of similarly treated cells grown on millipore filters. Applications of the method are discussed.

摘要

,,539 - 544。描述了一种评估同种异体移植灭菌程序对培养的人成纤维细胞活力影响的半定量方法。使用光学显微镜及其与中性红和黑色素的染色反应,估计盖玻片上四组成纤维细胞中“存活”、“改变”和“非存活”细胞的比例。虽然一组成纤维细胞对照组保持存活,但将其暴露于同种异体移植制备中使用的三种溶液中的每一种一至三周,会导致细胞逐渐从“改变”类别过渡到“非存活”类别。通过对在微孔滤膜上生长的经类似处理的细胞进行电子显微镜检查,证实了这些发现。讨论了该方法的应用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0eb6/470195/76119cb3fed3/thorax00137-0059-a.jpg

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