通过抗体标记亚基的电子显微镜观察对大肠杆菌核糖体蛋白S4和S14进行定位
Localization of Escherichia coli ribosomal proteins S4 and S14 by electron microscopy of antibody-labeled subunits.
作者信息
Lake J A, Pendergast M, Kahan L, Nomura M
出版信息
Proc Natl Acad Sci U S A. 1974 Dec;71(12):4688-92. doi: 10.1073/pnas.71.12.4688.
Binding sites for antibodies specific for proteins S4 and S14 of the small subunit of E. coli ribosomes have been mapped on the surface of the subunit by electron microscopy. Antibody binding to reconstituted subunits was shown to depend specifically on the presence of E. coli S4 and S14. Anti-S14 IgG was found to bind to a limited region of the ribosome surface. In contrast anti-S4 IgG was found to bind to three separated regions of the ribosome surface, suggesting S4 has an elongated conformation in situ.
通过电子显微镜已将针对大肠杆菌核糖体小亚基蛋白质S4和S14的特异性抗体的结合位点定位在该亚基的表面。抗体与重组亚基的结合被证明特别依赖于大肠杆菌S4和S14的存在。发现抗S14 IgG结合于核糖体表面的一个有限区域。相比之下，发现抗S4 IgG结合于核糖体表面的三个分离区域，这表明S4在原位具有伸长的构象。
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