Orlova T G, Golovanova T A, Shcheglovitova O N, Mysyakin E B, Bokhonko A I, Kaulen D R
Acta Virol. 1979 May;23(3):177-82.
Pretreatment with crude interferon preparations obtained from suspension cultures of bone marrow, spleen and thymus cells or from mouse L-cell cultures or with mouse serum interferon preparations did not change the colony-forming activity of bone marrow cells on syngeneic transplantation to lethally irradiated mice. Preparations of L-cell culture interferon, dialysed and purified by carboxymethyl-Sephadex (G-25) column chromatography, showed an inhibitory effect on exogenous colony formation by bone marrow cells. The results suggested the presence in crude interferon preparations of a substance either inhibiting the anticellular effect of interferon or stimulating colony formation. The factor produced by thymus cells following their treatment with antilymphocyte serum inhibited colony formation by bone marrow cells and, unlike interferon, possessed no antiviral activity when tested in cell cultures.
用从骨髓、脾脏和胸腺细胞悬浮培养物或小鼠L细胞培养物中获得的粗制干扰素制剂，或用小鼠血清干扰素制剂进行预处理，并不会改变骨髓细胞在同基因移植到致死性照射小鼠后形成集落的活性。经羧甲基-葡聚糖凝胶（G-25）柱色谱透析和纯化的L细胞培养干扰素制剂，对骨髓细胞的外源性集落形成显示出抑制作用。结果表明，粗制干扰素制剂中存在一种物质，它要么抑制干扰素的抗细胞作用，要么刺激集落形成。胸腺细胞在用抗淋巴细胞血清处理后产生的因子抑制骨髓细胞的集落形成，并且与干扰素不同，在细胞培养中检测时不具有抗病毒活性。
