Man'ko V M, Choladze N V, Guseva O A, Sepiashvili R I, Iarilin A A
Tsitologiia. 1996;38(1):48-56.
The hemopoietic stem cells (HSC), obtained from the mouse bone marrow and transferred into lethally irradiated syngeneic mice, do not form colonies of hemopoietic cells in the spleen of recipients. The colony formation has been observed after transplantation of HSC together with bone marrow B-lymphocytes or precursors of T-lymphocytes (PTL), and has been mediated through soluble products of nonimmunoglobulin nature produced by B-cells. In distinction from bone marrow B-cells, the interaction of splenic B-lymphocytes with the HSC fraction does not initiate this process, but suppresses it in the presence of bone marrow PTL. The interaction of B-lymphocytes activated by LPS (in vitro) or sheep erythrocytes (in vivo) with HSC or with a mixture of HSC and PTL resulted in the formation of splenic colonies. However, the induction of colony formation has been observed only in the presence of mature T-lymphocytes (phenotype Thy-1, Sc-1), revealed as an admixture to HSC, and has been abolished after their removing in the process of fractionation of bone marrow and receiving the purified HSC fraction. It requires some membranous interaction of regulatory cells.
从小鼠骨髓中获取并移植到接受致死剂量照射的同基因小鼠体内的造血干细胞（HSC），在受体小鼠的脾脏中不会形成造血细胞集落。在将HSC与骨髓B淋巴细胞或T淋巴细胞前体（PTL）一起移植后，观察到了集落形成，并且这一过程是由B细胞产生的非免疫球蛋白性质的可溶性产物介导的。与骨髓B细胞不同，脾B淋巴细胞与HSC部分的相互作用不会启动这一过程，而是在存在骨髓PTL的情况下抑制它。经脂多糖（体外）或绵羊红细胞（体内）激活的B淋巴细胞与HSC或HSC与PTL的混合物相互作用，会导致脾集落的形成。然而，仅在存在成熟T淋巴细胞（表型为Thy-1、Sc-1）的情况下观察到集落形成的诱导，这些成熟T淋巴细胞作为HSC的混合物被发现，并且在骨髓分级分离过程中去除它们并获得纯化的HSC部分后，集落形成的诱导被消除。这需要调节细胞的一些膜相互作用。
