Nakagawara G, Yamasaki G, Kimura S, Kojima Y, Miyazaki I
Surgery. 1978 Feb;83(2):188-93.
Preserving pancreatic islets for 7 days by a making use of the organ culture, we studied the insulin-releasing activity at the time of administration of glucose and various digestive tract hormones for the purpose of clarifying the function of preserved pancreatic islets. Furthermore, we transplanted pancreatic islets preserved for 3 to 5 days into the portal vein of rats with streptozotocin-induced diabetes and reached the following conclusions: (1). The islets of Langerhans of the pancreas responded well to glucose up to the seventh day of preservation and showed patterns similar to those of fresh pancreatic islets with respect to both the dose response and the time response. (2). Preserved pancreatic islets of the pancreas had insulin-releasing activity almost equal to that of fresh pancreatic islets against stimulation by glucagon, tolbutamide, and various digestive tract hormones. (3). Rats with streptozotocin-induced diabetes showed a marked improvement in blood glucose and urine glucose following transplantation of preserved pancreatic islets into the portal vein, and this effectiveness persisted for 6 to 8 weeks.
我们通过器官培养将胰岛保存7天，为阐明保存的胰岛功能，研究了给予葡萄糖和各种消化道激素时的胰岛素释放活性。此外，我们将保存3至5天的胰岛移植到链脲佐菌素诱导的糖尿病大鼠的门静脉中，得出以下结论：（1）胰腺胰岛在保存的第7天对葡萄糖反应良好，在剂量反应和时间反应方面均表现出与新鲜胰岛相似的模式。（2）保存的胰腺胰岛对胰高血糖素、甲苯磺丁脲和各种消化道激素刺激的胰岛素释放活性几乎与新鲜胰岛相同。（3）将保存的胰岛移植到门静脉后，链脲佐菌素诱导的糖尿病大鼠的血糖和尿糖有明显改善，且这种效果持续6至8周。
