Nakagawara G, Kojima Y, Mizukami T, Ono H, Miyazaki I
Gastroenterol Jpn. 1981;16(5):514-20. doi: 10.1007/BF02774523.
pancreatic transplantation is the most effective therapy of advanced diabetes mellitus. However, the problem is whether the cadaver pancreas can be used as a donor, because the living pancreas cannot be used as a donor from an anatomical standpoint. In case of the cadaver pancreas another problem is coming up that the pancreas proceeds to rapid autolysis. So, brain death is recommended as the most available condition for this transplantation. In this study we investigated the viability of the cadaver pancreas from decapitated rats preserved by the cold perfusion method. The results obtained were as follows: 1) The perfusing time affected the ratio of degenerative islets/normal islets. A large number of degenerative islets appeared with long perfusion time. 2) The cold (4 degrees C) perfusion method could preserve cadaver pancreas for 5 hours. 3) Insulin-secretion of cadaver pancreatic islets preserved for 5 hours under cold perfusion was 60% or more of that of normal pancreatic islets. 4) Three hundred islets from cadaver pancreas preserved for 5 hours under cold perfusion were transplanted into the portal vein of streptozotocin-induced diabetic rats. Four of these 11 rats recovered from diabetic state and maintained the non-diabetic state for 20 weeks after transplantation.
胰腺移植是晚期糖尿病最有效的治疗方法。然而，问题在于尸体胰腺能否用作供体，因为从解剖学角度来看，活体胰腺不能用作供体。对于尸体胰腺，另一个问题出现了，即胰腺会迅速自溶。因此，脑死亡被认为是这种移植最可行的条件。在本研究中，我们研究了通过冷灌注法保存的断头大鼠尸体胰腺的活力。获得的结果如下：1）灌注时间影响退化胰岛/正常胰岛的比例。灌注时间长会出现大量退化胰岛。2）冷（4摄氏度）灌注法可将尸体胰腺保存5小时。3）在冷灌注下保存5小时的尸体胰腺胰岛的胰岛素分泌量为正常胰腺胰岛的60%或更多。4）将在冷灌注下保存5小时的尸体胰腺的300个胰岛移植到链脲佐菌素诱导的糖尿病大鼠的门静脉中。这11只大鼠中有4只从糖尿病状态恢复，并在移植后20周维持非糖尿病状态。
