利用玻璃珠柱分离大鼠胸腺来源和骨髓来源的淋巴细胞。
Separation of thymus-derived and marrow-derived rat lymphocytes on glass bead columns.
作者信息
Hunt S V
出版信息
Immunology. 1973 Apr;24(4):699-705.
Rat thoracic duct lymphocytes were passed through columns of fine siliconed glass beads according to the method of Shortman (1966). On the basis of the degree of labelling with uridine and of surface antigenic markers it was shown that marrow-derived small lymphocytes were preferentially retained on the columns, causing the concentration of thymus-derived small lymphocytes in the filtered cells. Filtered cells retained normal graft-versus-host (GVH) activity. The proportion of thymus-derived small lymphocytes in normal rat lymph was estimated at a maximum of 63–87 per cent. Previous reports on the immunocompetence of filtered cells may readily be explained by these findings.
按照肖特曼（1966年）的方法，将大鼠胸导管淋巴细胞通过装有精细硅化玻璃珠的柱体。根据尿苷标记程度和表面抗原标志物表明，骨髓来源的小淋巴细胞优先保留在柱体上，从而使过滤后的细胞中胸腺来源的小淋巴细胞得以富集。过滤后的细胞保留了正常的移植物抗宿主（GVH）活性。正常大鼠淋巴中胸腺来源的小淋巴细胞比例估计最高为63% - 87%。先前关于过滤后细胞免疫活性的报道很容易用这些发现来解释。
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