通过玻璃珠柱分离的抗体形成细胞群体的异质性。
Heterogeneity of populations of antibody-forming cells fractionated by glass bead columns.
作者信息
Salerno A, Pontieri G M
出版信息
Clin Exp Immunol. 1969 Jul;5(1):209-12.
Spleen cell suspension, obtained from mice immunized 2–4 days previously with sheep erythrocytes, were filtered on glass bead columns. The proportion of cells which adhered to the column rose during the first 3 days to about 45%, compared with 8% in controls. This adherent fraction was shown to be relatively rich in antibody-forming cells as judged by immunocyto-adherence. After transfer to lethally irradiated recipients, cells of this fraction formed antibody which was detectable in the serum from 24 hours onwards. Cells of the non-adherent fraction also form antibody in irradiated recipients, but not before the 4th day after transfer.
从2 - 4天前用绵羊红细胞免疫的小鼠中获得的脾细胞悬液，通过玻璃珠柱进行过滤。与对照组的8%相比，在最初3天内，黏附于柱上的细胞比例上升至约45%。通过免疫细胞黏附判断，该黏附部分显示出相对富含抗体形成细胞。转移至经致死剂量照射的受体后，该部分细胞形成的抗体从24小时起即可在血清中检测到。非黏附部分的细胞在照射受体中也能形成抗体，但在转移后第4天才开始。
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