Zinder S H, Brock T D
Arch Microbiol. 1978 Jan 23;116(1):35-40. doi: 10.1007/BF00408731.
The isolation from lake mud of a bacterium which can use dimethyl sulfoxide (DMSO) as an electron acceptor for growth is described. The isolate, called strain DL-1, was a small, gram negative, non-motile spiral. The sole product of DMSO reduction was dimethyl sulfide (DMS). Other electron acceptors used by the isolate included sulfite, thiosulfate, elemental sulfur, methionine sulfoxide, tetramethylene sulfoxide, nitrate, and oxygen (microaerophilically). Sulfate was not reduced and could not even be assimilated. Lactate or succinate could serve as electron donors, with acetate as the main product. Hydrogen could be used as an electron donor if acetate was present in the medium as a carbon source. The organism has a c-type cytochrome, and most likely uses electron transport phosphorylation during DMSO reduction. Cultures of Desulfovibrio sp., Escherichia coli, Pseudomonas aeruginosa, and Proteus vulgaris were tested for growth using DMSO as an electron acceptor, and only the Proteus strain grew. Both Proteus and strain DL-1 are versatile at coupling reductions with energy generation. There is a marked resemblance between strain DL-1 and the recently described sulfur-reducing spirillum of Wolfe and Pfennig.
本文描述了从湖泥中分离出一种能利用二甲基亚砜（DMSO）作为生长电子受体的细菌。该分离菌株名为DL-1，是一种小型、革兰氏阴性、无运动性的螺旋菌。DMSO还原的唯一产物是二甲基硫醚（DMS）。该分离菌株使用的其他电子受体包括亚硫酸盐、硫代硫酸盐、元素硫、甲硫氨酸亚砜、四亚甲基亚砜、硝酸盐和氧气（微需氧条件下）。硫酸盐既不被还原，甚至也不能被同化。乳酸盐或琥珀酸盐可作为电子供体，主要产物为乙酸盐。如果培养基中存在乙酸盐作为碳源，氢气也可作为电子供体。该生物体含有一种c型细胞色素，在DMSO还原过程中很可能利用电子传递磷酸化。测试了脱硫弧菌属、大肠杆菌、铜绿假单胞菌和普通变形杆菌的培养物以DMSO作为电子受体时的生长情况，只有变形杆菌菌株能够生长。变形杆菌和DL-1菌株在将还原作用与能量产生相偶联方面都具有多样性。DL-1菌株与最近描述的Wolfe和Pfennig的硫还原螺旋菌之间存在明显的相似性。
