Lorenzen J, Steinwachs S, Unden G
Institut für Biochemie, Heinrich-Heine-Universität Düsseldorf, Germany.
Arch Microbiol. 1994;162(4):277-81. doi: 10.1007/BF00301851.
The anaerobic rumen bacterium Wolinella succinogenes was able to grow by respiration with dimethylsulphoxide (DMSO) as electron acceptor and formate or H2 as electron donors. The growth yield amounted to 6.7 g and 6.4 g dry cells/mol DMSO with formate or H2 as the donors, respectively. This suggested an ATP yield of about 0.7 mol ATP/mol DMSO. Cell homogenates and the membrane fraction contained DMSO reductase activity with a high Km (43 mM) for DMSO. The electron transport from H2 to DMSO in the membranes was inhibited by 2-(heptyl)-4-hydroxyquinoline N-oxide, indicating the participation of menaquinone. Formation of DMSO reductase activity occurred only during growth on DMSO, presence of other electron acceptors (fumarate, nitrate, nitrite, N2O, and sulphur) repressed the DMSO reductase activity. DMSO can therefore be used by W. succinogenes as an acceptor for phosphorylative electron transport, but other electron acceptors are used preferentially.
厌氧瘤胃细菌琥珀酸沃氏菌能够以二甲基亚砜（DMSO）作为电子受体，以甲酸盐或H2作为电子供体通过呼吸作用生长。以甲酸盐或H2作为供体时，生长产量分别为每摩尔DMSO产生6.7克和6.4克干细胞。这表明每摩尔DMSO的ATP产量约为0.7摩尔ATP。细胞匀浆和膜部分含有对DMSO具有高Km（43 mM）的DMSO还原酶活性。膜中从H2到DMSO的电子传递受到2-（庚基）-4-羟基喹啉N-氧化物的抑制，表明甲基萘醌参与其中。DMSO还原酶活性仅在以DMSO为底物生长期间形成，其他电子受体（富马酸盐、硝酸盐、亚硝酸盐、N2O和硫）的存在会抑制DMSO还原酶活性。因此，琥珀酸沃氏菌可以将DMSO用作磷酸化电子传递的受体，但优先使用其他电子受体。
