零度以下微粒体中的酶活性测定。
Enzyme assay in microsomes below zero degrees.
作者信息
Debey P, Balny C, Douzou P
出版信息
Proc Natl Acad Sci U S A. 1973 Sep;70(9):2633-6. doi: 10.1073/pnas.70.9.2633.
Abstract
Reactions of a membrane-bound multienzyme complex (electron-transport chain of rat-liver microsomes) suspended in aqueous-organic solvent used as antifreeze at temperatures below 0 degrees were studied. In the presence of a relatively high concentration of ethylene glycol, electron transfer can still be observed and some individual and sequential enzyme assays can be performed over a wide range of temperatures below 0 degrees .
摘要
研究了悬浮于用作防冻剂的水-有机溶剂中、温度低于0摄氏度时的膜结合多酶复合物（大鼠肝脏微粒体电子传递链）的反应。在相对高浓度的乙二醇存在下，仍可观察到电子转移，并且在低于0摄氏度的很宽温度范围内可进行一些单独的和连续的酶分析。
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