Zeigler Z, Murphy S, Gardner F H
Blood. 1978 Mar;51(3):479-86.
Microscopic evaluation of apparent platelet size and morphology was examined in a variety of hematologic disorders. The time of preparation of the blood smear was important. An artifactual increase in platelet size was noted on blood films from 20 normal individuals that were prepared either immediately or 180 min after venipuncture. The clearest differentiation of patient categories was obtained with smears prepared 60 min after venipuncture using blood anticoagulated with K3EDTA. Under these conditions, normal size and morphology values were found in thrombocytopenic patients with aplasia or with increased splenic pooling. In contrast, large size values were a reliable finding in idiopathic thrombocytopenic purpura patients, whose platelet counts were less than 50,000/microleter. Large size values were also noted in patients with infiltrated bone marrows or myeloproliferative syndromes regardless of the platelet count. The last two groups usually showed abnormal platelet morphology with greater than 10% hypogranular platelets. Normal platelet size and morphology were observed in patients with iron-deficiency and megaloblastic anemias and in patients with idiopathic thrombocytopenic purpura and systemic lupus erythematosus who had normal platelet counts.
在多种血液系统疾病中对血小板的表观大小和形态进行了显微镜评估。血涂片的制备时间很重要。在20名正常个体的血涂片上发现，无论是在静脉穿刺后立即制备还是在180分钟后制备，血小板大小都会出现人为增加。使用K3EDTA抗凝的血液，在静脉穿刺60分钟后制备的涂片对患者类别有最清晰的区分。在这些条件下，再生障碍性贫血或脾池增加的血小板减少症患者的血小板大小和形态值正常。相比之下，血小板计数低于50,000/微升的特发性血小板减少性紫癜患者中，血小板大是一个可靠的发现。骨髓浸润或骨髓增殖综合征患者无论血小板计数如何也会出现血小板大。后两组通常表现出血小板形态异常，颗粒减少的血小板超过10%。缺铁性贫血和巨幼细胞贫血患者以及血小板计数正常的特发性血小板减少性紫癜和系统性红斑狼疮患者的血小板大小和形态正常。
