Lawford H G, Haddock B A
Biochem J. 1973 Sep;136(1):217-20. doi: 10.1042/bj1360217.
Measurements were made of the stoicheiometry of respiration-driven proton translocation coupled to the oxidation of NAD(P)-linked or flavin-linked substrates in intact cells of Escherichia coli. Observed stoicheiometries (-->H(+)/O quotient; Mitchell, 1966) were approx. 4 with l-malate as substrate and approx. 2 for succinate, d-lactate and glycerol oxidation. It is concluded that the potential number of equivalent energy-conservation sites associated with the respiratory chain is 2 in aerobically grown cells of E. coli harvested during the exponential phase of growth.
对大肠杆菌完整细胞中与NAD(P)连接或黄素连接底物氧化相偶联的呼吸驱动质子转运的化学计量进行了测量。观察到的化学计量(H⁺/O商;Mitchell,1966)以L-苹果酸为底物时约为4,琥珀酸、D-乳酸和甘油氧化时约为2。得出的结论是,在指数生长期收获的需氧生长的大肠杆菌细胞中,与呼吸链相关的等效能量保守位点的潜在数量为2。