通过阿的平荧光猝灭证明大肠杆菌电子传递缺陷型突变体膜中功能性呼吸链的重建。
The reconstitution of functional respiratory chains in membranes from electron-transport-deficient mutants of Escherichia coli as demonstrated by quenching of atebrin fluorescence.
作者信息
Haddock B A, Downie J A
出版信息
Biochem J. 1974 Sep;142(3):703-6. doi: 10.1042/bj1420703.
Abstract
Measurements were made of electron-transport-dependent quenching of atebrin fluorescence in particles prepared from mutants of Escherichia coli unable to synthesize either ubiquinone or haem. Such quenching was either absent (in haem-deficient particles) or decreased (in ubiquinone-deficient particles), but was restored under conditions used to reconstitute oxidase activities. It is concluded that the reconstitution of oxidase activity, in both cases, is associated with the formation of a functional, proton-translocating, respiratory chain.
摘要
对由无法合成泛醌或血红素的大肠杆菌突变体制备的颗粒中阿的平荧光的电子传递依赖性猝灭进行了测量。这种猝灭要么不存在（在血红素缺陷颗粒中），要么降低（在泛醌缺陷颗粒中），但在用于重建氧化酶活性的条件下得以恢复。得出的结论是，在这两种情况下，氧化酶活性的重建都与功能性的、质子转运的呼吸链的形成有关。
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