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1
The reconstitution of functional respiratory chains in membranes from electron-transport-deficient mutants of Escherichia coli as demonstrated by quenching of atebrin fluorescence.通过阿的平荧光猝灭证明大肠杆菌电子传递缺陷型突变体膜中功能性呼吸链的重建。
Biochem J. 1974 Sep;142(3):703-6. doi: 10.1042/bj1420703.
2
Functional anaerobic electron transport linked to the reduction of nitrate and fumarate in membranes from Escherichia coli as demonstrated by quenching of atebrin fluorescence.通过阿的平荧光猝灭证明，功能性厌氧电子传递与大肠杆菌膜中硝酸盐和富马酸盐的还原有关。
Biochem J. 1975 Dec;152(3):655-9. doi: 10.1042/bj1520655.
3
Energy-linked reduction of nicotinamide--adenine dinucleotide in membranes derived from normal and various respiratory-deficient mutant strains of Escherichia coli K12.来自大肠杆菌K12正常菌株及各种呼吸缺陷型突变菌株的膜中烟酰胺腺嘌呤二核苷酸的能量偶联还原作用
Biochem J. 1974 Oct;144(1):77-85. doi: 10.1042/bj1440077.
4
Energy transduction in Escherichia coli. The effect of chaotropic agents on energy coupling in everted membrane vesicles from aerobic and anaerobic cultures.大肠杆菌中的能量转换。离液剂对需氧和厌氧培养物外翻膜囊泡中能量偶联的影响。
Biochim Biophys Acta. 1977 Feb 7;459(2):225-40. doi: 10.1016/0005-2728(77)90024-x.
5
The function of ubiquinone in Escherichia coli.泛醌在大肠杆菌中的功能。
Biochem J. 1970 Apr;117(3):551-62. doi: 10.1042/bj1170551.
6
Anomalous effect of uncouplers on respiratory chain-linked transhydrogenation in Escherichia coli membranes: evidence for a localized proton pathway?解偶联剂对大肠杆菌膜中呼吸链偶联转氢作用的异常效应：局部质子途径的证据？
Arch Biochem Biophys. 1992 Mar;293(2):246-53. doi: 10.1016/0003-9861(92)90392-a.
7
Studies on electron transport and energy-linked reactions using mutants of Escherichia coli.利用大肠杆菌突变体进行的电子传递和能量偶联反应研究。
Biochim Biophys Acta. 1974 Apr 30;346(1):1-25. doi: 10.1016/0304-4173(74)90010-x.
8
Ascorbate-phenazine methosulfate-dependent membrane energization in respiratory chain mutants of Escherichia coli.大肠杆菌呼吸链突变体中抗坏血酸盐-吩嗪硫酸甲酯依赖性膜能量化作用
Biochem Biophys Res Commun. 1976 Sep 7;72(1):195-201. doi: 10.1016/0006-291x(76)90979-7.
9
Ubiquinone deficiency in an auxotroph of Escherichia coli requiring 4-hydroxybenzoic acid.在需要4-羟基苯甲酸的大肠杆菌营养缺陷型中泛醌缺乏。
Biochem J. 1967 Jun;103(3):714-9. doi: 10.1042/bj1030714.
10
Energy transduction in Escherichia coli. The role of the Mg2+ATPase.大肠杆菌中的能量转导。Mg2+ATP酶的作用。
J Biol Chem. 1975 Nov 10;250(21):8409-15.

引用本文的文献

1
CO-Releasing Molecules Have Nonheme Targets in Bacteria: Transcriptomic, Mathematical Modeling and Biochemical Analyses of CORM-3 [Ru(CO)3Cl(glycinate)] Actions on a Heme-Deficient Mutant of Escherichia coli.一氧化碳释放分子在细菌中有非血红素靶点：CORM-3 [Ru(CO)3Cl(甘氨酸盐)] 对大肠杆菌血红素缺陷突变体作用的转录组学、数学建模及生化分析
Antioxid Redox Signal. 2015 Jul 10;23(2):148-62. doi: 10.1089/ars.2014.6151. Epub 2015 Apr 28.
2
An amino-acid substitution in the influenza-B NB protein affects ion-channel gating.乙型流感病毒NB蛋白中的氨基酸替换影响离子通道门控。
J Membr Biol. 2004 Feb 1;197(3):135-43. doi: 10.1007/s00232-004-0648-0.
3
The Vpu protein of human immunodeficiency virus type 1 forms cation-selective ion channels.1型人类免疫缺陷病毒的Vpu蛋白形成阳离子选择性离子通道。
J Virol. 1996 Oct;70(10):7108-15. doi: 10.1128/JVI.70.10.7108-7115.1996.
4
Assembly of the adenosine triphosphatase complex in Escherichia coli: assembly of F0 is dependent on the formation of specific F1 subunits.大肠杆菌中三磷酸腺苷酶复合物的组装：F0 的组装依赖于特定 F1 亚基的形成。
J Bacteriol. 1981 Oct;148(1):30-42. doi: 10.1128/jb.148.1.30-42.1981.
5
Three genes coding for subunits of the membrane sector (F0) of the Escherichia coli adenosine triphosphatase complex.编码大肠杆菌三磷酸腺苷酶复合体膜部（F0）亚基的三个基因。
J Bacteriol. 1981 Jan;145(1):200-10. doi: 10.1128/jb.145.1.200-210.1981.
6
The respiratory chains of Escherichia coli.大肠杆菌的呼吸链
Microbiol Rev. 1984 Sep;48(3):222-71. doi: 10.1128/mr.48.3.222-271.1984.
7
The mechanism of proton translocation driven by the respiratory nitrate reductase complex of Escherichia coli.大肠杆菌呼吸硝酸盐还原酶复合物驱动质子转运的机制。
Biochem J. 1980 Jul 15;190(1):79-94. doi: 10.1042/bj1900079.
8
Proton translocation in intack cells of Thiobacillus denitrificans.反硝化硫杆菌完整细胞中的质子转运
Arch Microbiol. 1977 May 13;113(1-2):11-6. doi: 10.1007/BF00428573.
9
Functional anaerobic electron transport linked to the reduction of nitrate and fumarate in membranes from Escherichia coli as demonstrated by quenching of atebrin fluorescence.通过阿的平荧光猝灭证明，功能性厌氧电子传递与大肠杆菌膜中硝酸盐和富马酸盐的还原有关。
Biochem J. 1975 Dec;152(3):655-9. doi: 10.1042/bj1520655.
10
Characterization of an Escherichia coli K12 mutant that is sensitive to chlorate when grown aerobically.一株需氧生长时对氯酸盐敏感的大肠杆菌K12突变体的特性分析。
Biochem J. 1978 Nov 15;176(2):553-61. doi: 10.1042/bj1760553.

本文引用的文献

1
uncoupling of oxidative phosphorylation by carbonyl cyanide phenylhydrazones. I. Some characteristics of m-Cl-CCP action on mitochondria and chloroplasts.羰基氰苯腙对氧化磷酸化的解偶联作用。I. 间氯苯腙对线粒体和叶绿体作用的一些特性。
Biochemistry. 1963 Mar-Apr;2:357-61. doi: 10.1021/bi00902a031.
2
Energy transduction in photosynthetic bacteria V. Role of coupling factor ATPase in energy conversion as revealed by light or ATP-induced quenching of atebrine fluorescence.光合细菌中的能量转换 V. 光或ATP诱导的阿的平荧光猝灭揭示偶联因子ATP酶在能量转换中的作用
FEBS Lett. 1972 Aug 1;24(2):141-145. doi: 10.1016/0014-5793(72)80752-x.
3
Oxidative phosphorylation in Azotobacter vinelandii. Atebrin as a fluorescent probe for the energized state.棕色固氮菌中的氧化磷酸化。阿的平作为能量化状态的荧光探针。
Biochim Biophys Acta. 1970 Aug 4;216(1):231-3. doi: 10.1016/0005-2728(70)90177-5.
4
Determination of pH in chloroplasts. 2. Fluorescent amines as a probe for the determination of pH in chloroplasts.叶绿体中pH值的测定。2. 荧光胺作为测定叶绿体中pH值的探针。
Eur J Biochem. 1972 Jan 31;25(1):64-70. doi: 10.1111/j.1432-1033.1972.tb01667.x.
5
Active transport and binding in mitochondria.线粒体中的主动运输与结合
Biochim Biophys Acta. 1973 Dec 31;301(3):195-226. doi: 10.1016/0304-4173(73)90004-9.
6
The membrane ATPase of Escherichia coli. I. Release into solution, allotopic properties and reconstitution of membrane-bound ATPase.大肠杆菌的膜ATP酶。I. 释放到溶液中、异位性质及膜结合ATP酶的重组。
Biochim Biophys Acta. 1973 May 30;305(2):249-59. doi: 10.1016/0005-2728(73)90173-4.
7
Electron-transport chains of Escherichia coli. Reconstitution of respiration in a 5-aminolaevulinic acid-requiring mutant.大肠杆菌的电子传递链。在一株需要5-氨基乙酰丙酸的突变体中对呼吸作用的重建。
Eur J Biochem. 1973 May;35(1):34-45. doi: 10.1111/j.1432-1033.1973.tb02806.x.
8
Function of ubiquinone in Escherichia coli: a mutant strain forming a low level of ubiquinone.泛醌在大肠杆菌中的功能：形成低水平泛醌的突变菌株
J Bacteriol. 1972 Jan;109(1):69-73. doi: 10.1128/jb.109.1.69-73.1972.
9
A comparison of mitochondria from Torulopsis utilis grown in continuous culture with glycerol, iron, ammonium, magnesium or phosphate as the growth-limiting nutrient.对在连续培养中以甘油、铁、铵、镁或磷酸盐作为生长限制营养物培养的产朊假丝酵母的线粒体进行比较。
Biochem J. 1971 Aug;124(1):123-34. doi: 10.1042/bj1240123.
10
Energy coupling of the -methylgalactoside transport system of Escherichia coli.大肠杆菌α-甲基半乳糖苷转运系统的能量偶联
J Biol Chem. 1973 Jun 25;248(12):4429-35.

通过阿的平荧光猝灭证明大肠杆菌电子传递缺陷型突变体膜中功能性呼吸链的重建。

The reconstitution of functional respiratory chains in membranes from electron-transport-deficient mutants of Escherichia coli as demonstrated by quenching of atebrin fluorescence.

作者信息

Haddock B A, Downie J A

出版信息

Biochem J. 1974 Sep;142(3):703-6. doi: 10.1042/bj1420703.

DOI:10.1042/bj1420703
PMID:4377217
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1168339/
Abstract

Measurements were made of electron-transport-dependent quenching of atebrin fluorescence in particles prepared from mutants of Escherichia coli unable to synthesize either ubiquinone or haem. Such quenching was either absent (in haem-deficient particles) or decreased (in ubiquinone-deficient particles), but was restored under conditions used to reconstitute oxidase activities. It is concluded that the reconstitution of oxidase activity, in both cases, is associated with the formation of a functional, proton-translocating, respiratory chain.

摘要

对由无法合成泛醌或血红素的大肠杆菌突变体制备的颗粒中阿的平荧光的电子传递依赖性猝灭进行了测量。这种猝灭要么不存在（在血红素缺陷颗粒中），要么降低（在泛醌缺陷颗粒中），但在用于重建氧化酶活性的条件下得以恢复。得出的结论是，在这两种情况下，氧化酶活性的重建都与功能性的、质子转运的呼吸链的形成有关。