Griffin F M, Silverstein S C
J Exp Med. 1974 Feb 1;139(2):323-36. doi: 10.1084/jem.139.2.323.
A method of attaching mouse RBCs to mouse macrophages is described. Both cell types were coated with rabbit anti-mouse macrophage F(ab')(2), and cross-linkage of cells was effected with sheep F(ab')(2) directed against rabbit F(ab')(2). 98% of macrophages attached an average of 11 RBCs each. Attachment occurred at 37 degrees C and was stable for at least 4 h. Less than 0.1% of macrophages ingested RBCs under these conditions. Latex particles and opsonized pneumococci were ingested as avidly by RBC-coated macrophages as by native macrophages. Ingestion of these particles did not prompt ingestion of attached RBCs. When anti-RBC IgG was added, however, over 90% of macrophages ingested an average of six RBCs each. Thus, ingestion of one particle does not trigger generalized phagocytosis of all particles attached to the cell's plasma membrane, and the phagocytic stimulus is confined to the segment of the cell's plasma membrane immediately adjacent to the particle being ingested.
描述了一种将小鼠红细胞附着于小鼠巨噬细胞的方法。两种细胞类型均用兔抗小鼠巨噬细胞F(ab')(2)包被，细胞的交联用针对兔F(ab')(2)的羊F(ab')(2)实现。98%的巨噬细胞平均每个附着11个红细胞。附着在37℃发生且至少4小时稳定。在这些条件下，不到0.1%的巨噬细胞摄取红细胞。红细胞包被的巨噬细胞摄取乳胶颗粒和调理的肺炎球菌的程度与天然巨噬细胞一样强烈。摄取这些颗粒并未促使摄取附着的红细胞。然而，当加入抗红细胞IgG时，超过90%的巨噬细胞平均每个摄取6个红细胞。因此，摄取一个颗粒不会引发附着于细胞膜上所有颗粒的全身性吞噬作用，吞噬刺激局限于紧邻正在摄取颗粒的细胞膜部分。
