COHN Z A, MORSE S I
J Exp Med. 1960 May 1;111(5):667-87. doi: 10.1084/jem.111.5.667.
The phagocytosis and intracellular destruction of bacteria by rabbit polymorphonuclear leucocytes has been studied in vitro under defined conditions. The efficient and continuing ingestion of bacteria was dependent upon (a) opsonic factors present in fresh rabbit serum as well as upon, (b) the availability of an adequate supply of glucose in the medium. The effects of selected enzymatic inhibitors on the metabolic and functional activities of the leucocytes was investigated. Cyanide which inhibited oxygen consumption had no effect on the ingestion or inactivation of bacteria, Iodoacetate and arsenite which blocked glycolysis produced a marked inhibition in particle ingestion. 2,4-Dinitrophenol which stimulated both oxygen consumption and glycolysis, depressed phagocytosis after a 1 hour latent period. It was concluded that phagocytosis was an energy-requiring process in which glycolysis served as the most important source of energy. Leucocytes which were ingesting heat-killed bacteria exhibited increases in oxygen consumption, glucose utilization, and lactic acid synthesis. The effect of particle ingestion on glycogen metabolism was characterized by an initial period of glycogenolysis followed by an enhanced rate of glycogen synthesis. Leucocytes which had previously ingested heat-killed bacteria also demonstrated increased rates of phagocytosis.
在特定条件下，对家兔多形核白细胞对细菌的吞噬作用及细胞内破坏作用进行了体外研究。细菌的有效持续摄取取决于：（a）新鲜家兔血清中存在的调理素因子，以及（b）培养基中充足葡萄糖的供应。研究了所选酶抑制剂对白细胞代谢和功能活性的影响。抑制氧消耗的氰化物对细菌的摄取或灭活没有影响，阻断糖酵解的碘乙酸盐和亚砷酸盐对颗粒摄取产生显著抑制。刺激氧消耗和糖酵解的2,4-二硝基苯酚在1小时潜伏期后抑制吞噬作用。得出结论，吞噬作用是一个需要能量的过程，其中糖酵解是最重要的能量来源。摄取热杀死细菌的白细胞表现出氧消耗、葡萄糖利用和乳酸合成增加。颗粒摄取对糖原代谢的影响表现为最初的糖原分解期，随后糖原合成速率加快。先前摄取过热杀死细菌的白细胞也表现出吞噬作用速率增加。
