Uhlig G, Kolb E
Arch Exp Veterinarmed. 1979;33(2):225-36.
The following parameters are recommended for activity determination of FDPase by release of inorganic phosphate at 37 degrees C of enzyme from liver and kidneys of swine: pH 7.5, 2 mMol concentration of fructose-1,6-diphosphate, 5 mMol concentration of Mg ions, and ten minutes of incubation. When the activity of FDPase was analysed with temperatures of 22 degrees C, and 45 degrees C, rises were recorded up to the highest temperature. ADTA was strongly activating along with 2 mMol of Mg ions in a test arrangement of 5 mMol concentration. Manganese, zinc, and cobalt ions are strong activators even in low concentrations (0.2 or 1.0 mMol), whereas copper, cadmium, and mercury ions are strong inhibitors of the enzyme. Average activities of FDPase were analysed by means of the optical test in the liver and kidneys of fetuses aged 98 days. They were 1.79 or 0.38 units in one gram of tissue. In pigs for slaughter they had been 2.06 or 3.58 units in one gram of tissue.
通过在37摄氏度下测定猪肝脏和肾脏中酶释放无机磷酸盐来测定果糖二磷酸酶（FDPase）活性时，建议采用以下参数：pH 7.5、果糖-1,6-二磷酸浓度为2毫摩尔、镁离子浓度为5毫摩尔以及孵育10分钟。当在22摄氏度和45摄氏度的温度下分析FDPase活性时，记录到活性随温度升高直至最高温度均有所上升。在浓度为5毫摩尔的测试体系中，乙二胺四乙酸（ADTA）与2毫摩尔镁离子一起具有强烈的激活作用。锰、锌和钴离子即使在低浓度（0.2或1.0毫摩尔）时也是强激活剂，而铜、镉和汞离子是该酶的强抑制剂。通过光学测试分析了98日龄胎儿肝脏和肾脏中FDPase的平均活性。每克组织中它们分别为1.79或0.38单位。在待屠宰猪中，每克组织中它们分别为2.06或3.58单位。
