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1
The effects of iron-limited growth on the reduced nicotinamide-adenine dinucleotide dehydrogenase activity and the membrane proteins of Candida utilis mitochondria.铁限制生长对产朊假丝酵母线粒体中还原型烟酰胺腺嘌呤二核苷酸脱氢酶活性及膜蛋白的影响。
Biochem J. 1973 Dec;136(4):1029-37. doi: 10.1042/bj1361029.
2
Piericiden A sensitivity, site 1 phosphorylation, and reduced nicotinamide adenine dinucleotide dehydrogenase during iron-limited growth of Candida utilis.产朊假丝酵母铁限制生长期间的杀稻瘟菌素A敏感性、位点1磷酸化和还原型烟酰胺腺嘌呤二核苷酸脱氢酶
J Biol Chem. 1975 Jan 10;250(1):211-7.
3
Electron-paramagnetic-resonance spectroscopy studies of iron-sulphur centres of submitochondrial particles from iron- and sulphur-deficient. Candida utilis.对来自缺铁和缺硫的产朊假丝酵母的亚线粒体颗粒中铁硫中心的电子顺磁共振光谱研究。
Biochem J. 1975 Jan;146(1):239-46. doi: 10.1042/bj1460239.
4
On the reaction of piericidin A with the reduced nicotinamide adenine dinucleotide dehydrogenase of Candida utilis.
J Biol Chem. 1974 Jun 25;249(12):3814-8.
5
Effect of sulphate-limited growth on mitochondrial electron transfer and energy conservation between reduced nicotinamide-adenine dinucleotide and the cytochromes in Torulopsis utilis.硫酸盐限制生长对产朊假丝酵母中还原型烟酰胺腺嘌呤二核苷酸与细胞色素之间线粒体电子传递和能量守恒的影响。
Biochem J. 1971 Aug;124(1):155-70. doi: 10.1042/bj1240155.
6
The purification and properties of the respiratory-chain reduced nicotinamide--adenine dinucleotide dehydrogenase of Torulopsis utilis.产朊假丝酵母呼吸链还原型烟酰胺腺嘌呤二核苷酸脱氢酶的纯化及性质
Biochem J. 1971 Oct;124(5):853-65. doi: 10.1042/bj1240853.
7
Spectroscopic studies of flavoproteins and non-haem iron proteins of submitochondrial particles of Torulopsis utilis modified by iron- and sulphate-limited growth in continuous culture.对在连续培养中受铁和硫酸盐限制生长所修饰的产朊假丝酵母亚线粒体颗粒的黄素蛋白和非血红素铁蛋白的光谱研究。
Biochem J. 1971 Aug;124(1):171-87. doi: 10.1042/bj1240171.
8
Non-haem iron and the dissociation of piericidin A sensitivity from site 1 energy conservation in mitochondria from Torulopsis utilis.产朊假丝酵母线粒体中，非血红素铁与抗霉素A敏感性从位点1能量守恒的解离
Biochem J. 1971 Aug;124(1):135-51. doi: 10.1042/bj1240135.
9
Reduced nicotinamide adenine dinucleotide dehydrogenase, piericidin sensitivity, and site 1 phosphorylation in different growth phases of Candida utilis.产朊假丝酵母不同生长阶段中烟酰胺腺嘌呤二核苷酸脱氢酶的减少、杀稻瘟菌素敏感性及位点1磷酸化
J Biol Chem. 1974 Jun 25;249(12):3819-26.
10
Studies on non-heme iron proteins and the piericidin A binding site of submitochondrial particles from Candida utilis cells grown in media of varying iron concentrations.对非血红素铁蛋白以及来自在不同铁浓度培养基中生长的产朊假丝酵母细胞的亚线粒体颗粒的抗霉素A结合位点的研究。
Biochem Biophys Res Commun. 1969 Aug 7;36(3):487-93. doi: 10.1016/0006-291x(69)90591-9.

引用本文的文献

1
Purification and some properties of nitrate reductase (EC 1.7.99.4) from Escherichia coli K12.来自大肠杆菌K12的硝酸还原酶（EC 1.7.99.4）的纯化及某些性质
Biochem J. 1976 Mar 1;153(3):533-41. doi: 10.1042/bj1530533.

本文引用的文献

1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Studies on the respiratory chain-linked dihydrodiphosphopyridine nucleotide dehydrogenase. I. Assay of the enzyme in particulate and in soluble preparations.呼吸链偶联二氢二磷酸吡啶核苷酸脱氢酶的研究。I. 颗粒制剂和可溶制剂中该酶的测定
J Biol Chem. 1962 Feb;237:569-76.
3
NITRATE REDUCTASE IN CELL-FREE EXTRACTS OF A HAEMIN-REQUIRING STRAIN OF STAPHYLOCOCCUS AUREUS.一株需要血红素的金黄色葡萄球菌无细胞提取物中的硝酸还原酶
Biochem J. 1963 Dec;89(3):503-10. doi: 10.1042/bj0890503.
4
[Formation of respiratory enzymes in streptomycin-resistant and heme-auxotrophic mutant of Escherichia coli].[大肠杆菌链霉素抗性及血红素营养缺陷型突变体中呼吸酶的形成]
Ann Inst Pasteur (Paris). 1957 Mar;92(3):396-412.
5
Inter-relationships between mitochondrial energy conservation at site I, piericidin a sensitivity, and EPR spectra in torulopsis utilis.产朊假丝酵母中位点I的线粒体能量守恒、抗霉素A敏感性与电子顺磁共振谱之间的相互关系。
FEBS Lett. 1969 Nov 12;5(3):207-210. doi: 10.1016/0014-5793(69)80333-9.
6
The oxidation-reduction potentials of iron-sulfur centers in the site I region of the respiratory chain in C. utilis submitochondrial particles.
FEBS Lett. 1972 Mar;21(1):59-62. doi: 10.1016/0014-5793(72)80163-7.
7
Iron-limited growth of torulopsis utilis, and the reversible loss of mitochondrial energy conservation at site 1 and of sensitivity to rotenone and piericidin A.
FEBS Lett. 1968 Jul;1(1):4-8. doi: 10.1016/0014-5793(68)80004-3.
8
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定分子量的可靠性。
J Biol Chem. 1969 Aug 25;244(16):4406-12.
9
A convenient method of preparing polyacrylamide gels for liquid scintillation spectrometry.一种制备用于液体闪烁光谱分析的聚丙烯酰胺凝胶的简便方法。
Anal Biochem. 1968 Jan;22(1):89-98. doi: 10.1016/0003-2697(68)90262-5.
10
Growth yields of Torulopsis utilis grown in continuous culture with glycerol or iron as the growth-limiting nutrient.以甘油或铁作为生长限制营养物，在连续培养中生长的产朊假丝酵母的生长产量。
Biochem J. 1971 Aug;124(1):152-4. doi: 10.1042/bj1240152.

铁限制生长对产朊假丝酵母线粒体中还原型烟酰胺腺嘌呤二核苷酸脱氢酶活性及膜蛋白的影响。

The effects of iron-limited growth on the reduced nicotinamide-adenine dinucleotide dehydrogenase activity and the membrane proteins of Candida utilis mitochondria.

作者信息

Clegg R A, Skyrme J E

出版信息

Biochem J. 1973 Dec;136(4):1029-37. doi: 10.1042/bj1361029.

DOI:10.1042/bj1361029
PMID:4150649
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1166053/
Abstract
  1. The mitochondrial NADH dehydrogenase (EC 1.6.99.3) of Candida utilis exhibited altered properties when the organism was grown under iron-limited conditions. No suitable acceptor was found for assay of this enzyme from iron-limited cells. 2. Mitochondrial membrane proteins from C. utilis were analysed by polyacrylamide-gel electrophoresis. Compared with glycerol-limited cells, iron limitation resulted in the loss of at least two polypeptides from the mitochondrial membrane. 3. Neither of the polypeptides affected by iron limitation was part of a cytochrome, although one of them was part of the mitochondrial NADH dehydrogenase. 4. Non-haem iron of mitochondrial membranes was released in the presence of sodium dodecyl sulphate, and electrophoresis in solutions of this detergent cannot be used directly to identify iron-sulphur proteins. Non-ionic detergents do not release non-haem iron but nor do they provide a satisfactory system for electrophoretic separation.
摘要
  1. 当产朊假丝酵母在铁限制条件下生长时，其线粒体NADH脱氢酶（EC 1.6.99.3）表现出性质改变。未找到合适的受体用于测定来自铁限制细胞的这种酶。2. 用聚丙烯酰胺凝胶电泳分析了产朊假丝酵母的线粒体膜蛋白。与甘油限制细胞相比，铁限制导致线粒体膜中至少两种多肽缺失。3. 受铁限制影响的两种多肽都不是细胞色素的一部分，尽管其中一种是线粒体NADH脱氢酶的一部分。4. 线粒体膜的非血红素铁在十二烷基硫酸钠存在下会释放出来，并且在这种去污剂溶液中的电泳不能直接用于鉴定铁硫蛋白。非离子去污剂不会释放非血红素铁，但它们也不能提供令人满意的电泳分离系统。