对来自缺铁和缺硫的产朊假丝酵母的亚线粒体颗粒中铁硫中心的电子顺磁共振光谱研究。

Electron-paramagnetic-resonance spectroscopy studies of iron-sulphur centres of submitochondrial particles from iron- and sulphur-deficient. Candida utilis.

Gray T A, Garland P B, Lowe D J, Garland P B

Biochem J. 1975 Jan;146(1):239-46. doi: 10.1042/bj1460239.

Abstract

Measurements were made at 12 degrees K of the electron-paramagnetic-resonance (e.p.r.) spectra of submitochondrial particles from Candida utilis cells grown under conditions that alter the amount of the mitochondrial NADH dehydrogenase (EC 1.6.99.3). 2. Iron-limited growth decreases the extent of iron-sulphur e.p.r. signals to undetectable values that are less than 1 percent of those normally found with glycerol-limited growth. 3. Small but significant signals attributable to the NADH dehydrogenase were detected in submitochondrial particles from sulphate-limited cells. 4. Measurements made on submitochondrial particles prepared from these and other phenotypically modified cells lead us to conclude that the presence of low-temperature e.p.r.-detectable iron-sulphur centres attributable to the NADH dehydrogenase are necessary but not sufficient for the coupling of ATP synthesis to the NADH dehydrogenase reaction in the mitochondrial membrane of C. utilis. 6. The amplitude of the g=2.01 signal observed in non-reduced submitochondrial particles is approximately tenfold diminished by iron limitation but not significantly altered by sulphate limitation.

摘要

在12K温度下，对产朊假丝酵母细胞的亚线粒体颗粒进行电子顺磁共振（e.p.r.）光谱测量，这些细胞在改变线粒体NADH脱氢酶（EC 1.6.99.3）含量的条件下生长。2. 铁限制生长会使铁硫e.p.r.信号强度降低到无法检测的值，该值不到甘油限制生长时正常信号强度的1%。3. 在硫酸盐限制细胞的亚线粒体颗粒中检测到了归因于NADH脱氢酶的微弱但显著的信号。4. 对由这些以及其他表型修饰细胞制备的亚线粒体颗粒进行测量后，我们得出结论：在产朊假丝酵母线粒体膜中，存在可通过低温e.p.r.检测到的、归因于NADH脱氢酶的铁硫中心，对于ATP合成与NADH脱氢酶反应的偶联是必要的，但不充分。6. 在未还原的亚线粒体颗粒中观察到的g = 2.01信号的幅度，因铁限制而大约降低了十倍，但因硫酸盐限制而没有显著改变。