[猪（Sus scrofa）胃黏膜中胃蛋白酶原-胃蛋白酶同工型的免疫化学特性]
[Immunochemical characteristics of pepsinogen-pepsin isoforms from the gastric mucosa of the pig, Sus scrofa].
作者信息
Kalinovskiĭ V P, Okulov V B
出版信息
Zh Evol Biokhim Fiziol. 1978 Jan-Feb;14(1):84-6.
PMID:415452
Abstract
Electrophoretic and immunoelectrophoretic studies reveal three isoforms of pepsinogen and pepsin which differ in their immunochemical properties. Activation of pepsinogen to pepsin at pH 2 does not affect antigenic properties of the isoforms of the enzyme. Some of the isoforms of commercial pepsin do not exhibit immunogenic properties, although reveal the enzymic activity. This finding indicates that immunochemical and biochemical determinants are located in different parts of isoenzyme molecules.
摘要
电泳和免疫电泳研究表明，胃蛋白酶原和胃蛋白酶有三种同工型，它们的免疫化学性质不同。在pH 2条件下胃蛋白酶原激活为胃蛋白酶并不影响该酶同工型的抗原特性。市售胃蛋白酶的一些同工型虽具有酶活性，但不表现出免疫原性。这一发现表明免疫化学和生化决定簇位于同工酶分子的不同部位。
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