从纤细裸藻中分离出的组蛋白的分级分离。
The fractionation of histones isolated from Euglena gracilis.
作者信息
Jardine N J, Leaver J L
出版信息
Biochem J. 1978 Jan 1;169(1):103-11. doi: 10.1042/bj1690103.
Abstract
- The histones of Euglena gracilis were separated by gel filtration into five fractions. 2. Each fraction was characterized in terms of its electrophoretic, solubility and compositional properties. 3. Euglena gracilis clearly contains histones corresponding to vertebrate H1, H2B, H3 and H4 fractions, although they all differ in containing more lysine. 4. The remaining Euglena histone is considered to be homologous to vertebrate histone H2A, but it differs in having a much higher ratio of lysine to arginine. 5. The Euglena histone H1 appears to be lacking in aspartic acid. 6. Electrophoresis in the presence of sodium dodecyl sulphate indicates that the molecular weights of the Euglena histones are close to those of the homologous vertebrate histones.
摘要
- 纤细裸藻的组蛋白通过凝胶过滤被分离成五个组分。2. 每个组分依据其电泳、溶解性和组成特性进行了表征。3. 纤细裸藻显然含有与脊椎动物H1、H2B、H3和H4组分相对应的组蛋白，尽管它们都含有更多赖氨酸，因而有所不同。4. 剩余的裸藻组蛋白被认为与脊椎动物组蛋白H2A同源，但它在赖氨酸与精氨酸的比例上要高得多。5. 裸藻组蛋白H1似乎缺乏天冬氨酸。6. 在十二烷基硫酸钠存在下进行的电泳表明，裸藻组蛋白的分子量与同源脊椎动物组蛋白的分子量相近。
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