用于电子显微镜检查的细胞骨架成分的保存。
Preservation of cytoskeletal elements for electron microscopy.
作者信息
Temmink J H, Spiele H
出版信息
Cell Biol Int Rep. 1978 Jan;2(1):51-9. doi: 10.1016/0309-1651(78)90084-x.
PMID:415818
Abstract
Attached murine fibroblasts were subjected to different fixation and dehydration procedures in order to study the preservation of cytoskeletal elements. The organization of the microtubular and microfilamentous system was identical whether or not post-fixed with osmic acid after glutaraldehyde fixation. Critical point drying did not preserve structures absent in conventionally dehydrated and embedded cells.
摘要
为了研究细胞骨架成分的保存情况，对贴壁的小鼠成纤维细胞进行了不同的固定和脱水处理。无论在戊二醛固定后是否用锇酸进行后固定，微管和微丝系统的组织结构都是相同的。临界点干燥并不能保存常规脱水和包埋细胞中不存在的结构。
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