Conservation of active ribosomes in acetone-treated cells of Tetrahymena pyriformis.
作者信息
Kristiansen K, Hartmann B, Plesner P, Krüger A
出版信息
Eur J Biochem. 1978 Feb;83(2):389-94. doi: 10.1111/j.1432-1033.1978.tb12104.x.
Abstract
The preparation of an acetone powder of cells of Tetrahymena pyriformis GL is described. A comparison of ribosomal particles isolated from acetone-treated and untreated cells shows that structurally and functionally intact ribosomes can be isolated from acetone-treated cells. Fully active ribosomes have been isolated from acetone powder of Tetrahymena that had been stored for more than 6 months at 4 degrees C. Thus, this procedure allows easy storage of large quantities of cells for the bulk preparation of active ribosomes.
摘要
相似文献
1
Conservation of active ribosomes in acetone-treated cells of Tetrahymena pyriformis.
Eur J Biochem. 1978 Feb;83(2):389-94. doi: 10.1111/j.1432-1033.1978.tb12104.x.
2
Ribosomal subunits from Tetrahymena pyriformis. Isolation and properties of active 40-S and 60-S subunits.梨形四膜虫的核糖体亚基。活性40-S和60-S亚基的分离与特性
Eur J Biochem. 1978 Sep 1;89(2):407-15. doi: 10.1111/j.1432-1033.1978.tb12543.x.
3
Strain-dependent variation of the protein composition of Tetrahymena ribosomes.
FEBS Lett. 1979 May 1;101(1):77-84.
4
Phosphorylation in vivo of Ribosomes in Tetrahymena pyriformis.梨形四膜虫核糖体的体内磷酸化作用
Eur J Biochem. 1978 Feb;83(2):395-403. doi: 10.1111/j.1432-1033.1978.tb12105.x.
5
Ribosome biosynthesis in Tetrahymena pyriformis. Regulation in response to nutritional changes.梨形四膜虫中的核糖体生物合成。对营养变化的响应调节。
J Cell Biol. 1976 Nov;71(2):383-94. doi: 10.1083/jcb.71.2.383.
6
A STEM approach to the study of the proteinaceous architecture of a ribosome.一种采用STEM方法对核糖体蛋白质结构进行的研究。
Biol Cell. 1984;50(3):247-54. doi: 10.1111/j.1768-322x.1984.tb00271.x.
7
Nonidentity of ribosomal structural proteins in growing and starved Tetrahymena.生长状态和饥饿状态的四膜虫中核糖体结构蛋白的非同一性
J Cell Biol. 1977 Oct;75(1):268-76. doi: 10.1083/jcb.75.1.268.
8
Isolation of subcellular membrane components from Tetrahymena.从四膜虫中分离亚细胞膜成分。
Methods Cell Biol. 1975;10:105-33.
9
Regulation of ribosome synthesis in Tetrahymena pyriformis. 3. Analysis by translation in vitro of RNA isolated during nutritional shift-down and nutritional shift-up.梨形四膜虫核糖体合成的调控。3. 营养下移和营养上移期间分离的RNA的体外翻译分析。
Eur J Biochem. 1984 May 2;140(3):485-92. doi: 10.1111/j.1432-1033.1984.tb08128.x.
10
Ribosomal proteins in growing and starved Tetrahymena pyriformis. Starvation-induced phosphorylation of ribosomal proteins.生长和饥饿状态下的梨形四膜虫中的核糖体蛋白。饥饿诱导的核糖体蛋白磷酸化。
Biochim Biophys Acta. 1978 Dec 21;521(2):435-51. doi: 10.1016/0005-2787(78)90285-x.
Zotero 插件