木瓜蛋白酶处理的球蛋白在特异性和交叉反应免疫荧光染色中的应用
Papain-treated globulins in specific and cross-reacting immunofluorescent staining.
作者信息
Jones W L, Foster J W
出版信息
J Bacteriol. 1966 Mar;91(3):984-6. doi: 10.1128/jb.91.3.984-986.1966.
Jones, Wallis L. (Communicable Disease Center, Atlanta, Ga.), and J. W. Foster. Papain-treated globulins in specific and cross-reacting immunofluorescent staining. J. Bacteriol. 91:984-986. 1966.-Cross-reaction staining with preimmune and specific fluorescein conjugates to Corynebacterium diphtheriae was studied by use of papain-treated antisera. Enzyme-treated antisera were labeled and chromatographically separated into three fractions. Fractions were tested by both direct and inhibition fluorescent-antibody procedures. The cross-reaction staining was found in all three fractions, whereas specific staining was found in only two fractions.
琼斯，沃利斯·L.（佐治亚州亚特兰大市传染病中心）和J.W.福斯特。木瓜蛋白酶处理的球蛋白在特异性和交叉反应免疫荧光染色中的应用。《细菌学杂志》91:984 - 986。1966年。——利用木瓜蛋白酶处理的抗血清研究了对白喉棒状杆菌的免疫前和特异性荧光素偶联物的交叉反应染色。酶处理的抗血清被标记并通过色谱法分离成三个组分。通过直接和抑制荧光抗体程序对各组分进行检测。在所有三个组分中均发现交叉反应染色，而仅在两个组分中发现特异性染色。
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