MOODY M D, JONES W L
J Bacteriol. 1963 Aug;86(2):285-93. doi: 10.1128/jb.86.2.285-293.1963.
Moody, Max D. (Communicable Disease Center, Atlanta, Ga.) and Wallis L. Jones. Identification of Corynebacterium diphtheriae with fluorescent antibacterial reagents. J. Bacteriol. 86:285-293. 1963.-Conditions are described whereby fluorescent-antibody reagents could be prepared and used to identify Corynebacterium diphtheriae in pure and mixed cultures and in clinical materials. The use of O and OK antigens for immunization of rabbits to prepare the antibody was compared. The most satisfactory reagents were those made from serum of rabbits injected with live (OK) suspensions of C. diphtheriae. Such fluorescent reagents were used successfully in direct and indirect fluorescent-antibody tests to identify both toxinogenic and atoxinogenic C. diphtheriae but not to differentiate the two kinds of organisms.
穆迪,马克斯·D.(佐治亚州亚特兰大传染病中心)和沃利斯·L.琼斯。用荧光抗菌试剂鉴定白喉棒状杆菌。《细菌学杂志》86:285 - 293。1963年。——本文描述了制备荧光抗体试剂并用于鉴定纯培养物、混合培养物及临床材料中的白喉棒状杆菌的条件。比较了使用O抗原和OK抗原免疫兔子制备抗体的情况。最令人满意的试剂是用注射了白喉棒状杆菌活(OK)悬液的兔子血清制成的。这种荧光试剂已成功用于直接和间接荧光抗体试验,以鉴定产毒素和不产毒素的白喉棒状杆菌,但不能区分这两种菌。