Herzberg M, Kenny K V, Robbins J B
J Bacteriol. 1966 Apr;91(4):1548-55. doi: 10.1128/jb.91.4.1548-1555.1966.
Herzberg, Mendel (University of Florida, Gainesville), Kathryn V. Kenny, and John B. Robbins. Nonabsorbable rabbit anti-Salmonella typhimurium antibody as detected by the complement-mediated bactericidal reaction. J. Bacteriol. 91:1548-1555. 1966.-A portion of antibody active in the complement-mediated bactericidal reaction against Salmonella typhimurium from hyperimmune rabbit serum has been shown to be nonabsorbable by repeated serial absorptions with whole heat-killed or living bacteria. The first two absorptions remove 90 to 95% of the activity, but 1 to 5% cannot be removed by subsequent absorptions. The nonabsorbable antibody appears to be a macroglobulin by density-gradient centrifugation and by comparison of activity and absorbability of purified gamma-M and gamma-G immune globulins. Alternative hypotheses involving low avidity antibody or antibody to minor cell antigenic components are offered in explanation of the phenomenon.
赫茨伯格，孟德尔（佛罗里达大学，盖恩斯维尔）、凯瑟琳·V·肯尼和约翰·B·罗宾斯。补体介导的杀菌反应检测到的不可吸收兔抗鼠伤寒沙门氏菌抗体。《细菌学杂志》91:1548 - 1555。1966年。——已证明，超免疫兔血清中对鼠伤寒沙门氏菌有补体介导杀菌反应活性的一部分抗体，经用全热灭活或活细菌反复连续吸收后不可吸收。前两次吸收去除了90%至95%的活性，但随后的吸收不能去除1%至5%的活性。通过密度梯度离心以及比较纯化的γ-M和γ-G免疫球蛋白的活性和吸收性，不可吸收抗体似乎是一种巨球蛋白。文中提出了涉及低亲和力抗体或针对次要细胞抗原成分的抗体的其他假说，以解释这一现象。
