Dolby J M, Dolby D E
Immunology. 1969 Jun;16(6):737-47.
antisera were fractionated on Sephadex G-200 to give 19S and 7S globulins, the latter refractionated on DEAE-cellulose, and the antibody properties of the two purified materials compared. The minimal weights of 19S and 7S globulins required to neutralize haemagglutinin and to protect mice against an intranasal challenge causing lung infection were similar. In agglutination 7S globulin was 1.5–100 times more effective than 19S, the ratio depending on strain and serum, but there was no apparent correlation with specific agglutinogens. 7S globulin was at least 100 times more effective than 19S both in the complement-dependent bactericidal reaction and in the protection of mice against an intracerebral challenge. It is suggested that the two reactions measure the same antibody.
将抗血清在葡聚糖凝胶G - 200上进行分级分离，得到19S和7S球蛋白，将后者在二乙氨基乙基纤维素上再进行分级分离，并比较这两种纯化物质的抗体特性。中和血凝素以及保护小鼠抵抗引起肺部感染的鼻内攻击所需的19S和7S球蛋白的最小重量相似。在凝集反应中，7S球蛋白的效力比19S球蛋白高1.5至100倍，该比例取决于菌株和血清，但与特异性凝集原没有明显相关性。在补体依赖性杀菌反应以及保护小鼠抵抗脑内攻击方面，7S球蛋白的效力至少比19S球蛋白高100倍。有人提出这两种反应检测的是同一种抗体。
