Dardas T J, Mallmann V H
J Bacteriol. 1966 Jul;92(1):76-81. doi: 10.1128/jb.92.1.76-81.1966.
Dardas, T. J. (Michigan State University, East Lansing), and V. H. Mallmann. Electrophoretic and immunoelectrophoretic studies of sera from normal, tuberculous, and noninfected tuberculin-sensitive guinea pigs. J. Bacteriol. 92:76-81. 1966.-Normal guinea pig serum was separated into seven fractions by electrophoresis on cellulose acetate membranes. Thirty antigens were found by immunoelectrophoresis: albumin, 6 alpha(1) globulins, 11 alpha(2) globulins, 6 beta(1) globulins, 5 beta(2) globulins, and gamma globulin. Hyper-alpha(2)-globulinemia was detected in sera from guinea pigs 14 days after inoculation with viable virulent Mycobacterium bovis. An additional alpha(2) globulin, not demonstrable prior to infection, was detected concomitantly with the hyper-alpha(2)-globulinemia by immunoelectrophoresis. The additional alpha(2) globulin was tentatively named alpha(2)-T. It persisted until the death of the guinea pigs. Neither hyper-alpha-globulinemia nor the alpha(2)-T antigen was detected by cellulose acetate electrophoresis and immunoelectrophoresis of sera from guinea pigs sensitized with heat-killed M. bovis. Both changes were due to the disease, not to delayed sensitivity alone.
达达什，T. J.（密歇根州立大学，东兰辛）和V. H. 马尔曼。正常、患结核病和未感染结核菌素敏感豚鼠血清的电泳和免疫电泳研究。《细菌学杂志》92:76 - 81。1966年。——通过在醋酸纤维素膜上进行电泳，将正常豚鼠血清分离成七个组分。通过免疫电泳发现了30种抗原：白蛋白、6种α(1)球蛋白、11种α(2)球蛋白、6种β(1)球蛋白、5种β(2)球蛋白和γ球蛋白。在用活的强毒牛分枝杆菌接种14天后的豚鼠血清中检测到α(2)球蛋白血症。通过免疫电泳在α(2)球蛋白血症的同时检测到一种在感染前未显示的额外α(2)球蛋白。这种额外的α(2)球蛋白被暂定为α(2)-T。它一直持续到豚鼠死亡。在用热灭活的牛分枝杆菌致敏的豚鼠血清的醋酸纤维素电泳和免疫电泳中，未检测到α球蛋白血症和α(2)-T抗原。这两种变化都是由疾病引起的，而不仅仅是由于迟发型超敏反应。
