绵羊垂体前叶中甘露糖基转移酶和岩藻糖基转移酶的特性分析。
Characterization of the mannosyl and fucosyl transferases in the ovine anterior pituitary glands.
作者信息
Tertrin-Clary C, De La Llosa-Hermier M P, De La Llosa P
出版信息
Biochimie. 1975;57(9):1073-7. doi: 10.1016/s0300-9084(75)80364-6.
PMID:4162
Abstract
Ovine anterior pituitary glands contain mannosyl- and fucosyl-transferases localized in the microsomes and able to incorporate mannose or fucose as such from GDP-mannose or GDP-fucose into endogenous glycoproteins. The requirements and conditions necessary for maximum activity were investigated. The value of the Km is very similar for the two enzyme systems, 3 X 10(-7) M in the case of mannosyl-transferases and 5 X 10(-7) M in the case of fucosyl-transferases.
摘要
绵羊脑垂体前叶含有定位在微粒体中的甘露糖基转移酶和岩藻糖基转移酶，它们能够将来自GDP-甘露糖或GDP-岩藻糖的甘露糖或岩藻糖原样掺入内源性糖蛋白中。研究了最大活性所需的条件。两种酶系统的Km值非常相似，甘露糖基转移酶的Km值为3×10^(-7)M，岩藻糖基转移酶的Km值为5×10^(-7)M。
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